Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2'-hydroxyl acylation of RNA

  • Chem Commun (Camb). 2014 Jun 1;50(43):5748-51. doi: 10.1039/c4cc01134a.
S Ursuegui  1 ,  N Chivot ,  S Moutin ,  A Burr ,  C Fossey ,  T Cailly ,  A Laayoun ,  F Fabis ,  A Laurent
Affiliations
  • 1. Normandie Univ, France.
PMID: 24752374 DOI: 10.1039/c4cc01134a
Abstract

An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA-RNA mixture, a selective 2'-hydroxyl acylation of RNAs followed by capture with streptavidin-coated Magnetic Beads and cleavage of the disulfide led to elution of RNAs.

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