1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme
  3. NSC232003

NSC232003 

Cat. No.: HY-103236 Purity: 98.66%
Handling Instructions

NSC232003 is a highly potent and cell-permeable UHRF1 inhibitor, which inhibits DNA methylation in vitro and disrupts DNMT1/UHRF1 interactions at a cellular level.

For research use only. We do not sell to patients.

NSC232003 Chemical Structure

NSC232003 Chemical Structure

CAS No. : 1905453-18-0

Size Price Stock Quantity
10 mM * 1 mL in Water USD 335 In-stock
Estimated Time of Arrival: December 31
1 mg USD 180 In-stock
Estimated Time of Arrival: December 31
5 mg USD 540 In-stock
Estimated Time of Arrival: December 31
10 mg USD 900 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2700 In-stock
Estimated Time of Arrival: December 31
100 mg USD 4020 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

NSC232003 is a highly potent and cell-permeable UHRF1 inhibitor, which inhibits DNA methylation in vitro and disrupts DNMT1/UHRF1 interactions at a cellular level.

IC50 & Target

UHRF1[1]

In Vitro

NSC232003, a uracil derivative freely available by the NCI/DTP repository, provides a versatile lead for developing highly potent and cell-permeable UHRF1 inhibitors that will enable dissection of DNA methylation inheritance. NSC232003 is indeed an effective DNA methylation inhibitor and indicate that this particular nucleotide scaffold could provide a versatile basis for the design of potent UHRF1 inhibitors. NSC232003 is predicted to be partially deprotonated at pH 7, as the pKa of the more acidic imide nitrogen of the pyrimidine ring is a value of 7.6 in NSC232003. The DNMT1/UHRF1 interactions are significantly reduced after 4 h of incubation of U251 glioma cells with the most potent compound NSC232003, showing a 50% interaction inhibition at 15 μM as well as induction of global DNA cytosine demethylation as measured by ELISA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

169.14

Formula

C₆H₇N₃O₃

CAS No.

1905453-18-0

SMILES

O=C1NC(/C(C=N1)=C(NO)/C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 17 mg/mL (100.51 mM; Need ultrasonic and warming)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9123 mL 29.5613 mL 59.1226 mL
5 mM 1.1825 mL 5.9123 mL 11.8245 mL
10 mM 0.5912 mL 2.9561 mL 5.9123 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 98.66%

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Keywords:

NSC232003NSC 232003NSC-232003E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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Product Name:
NSC232003
Cat. No.:
HY-103236
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