RTI-13951-33 hydrochloride

Name: RTI-13951-33 hydrochloride
Brand: MedChemExpress
SKU: HY-112612A
Rating: 4.5 (1 reviews)

Cat. No.: HY-112612A Purity: 98.56%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RTI-13951-33 hydrochloride is a potent, selective, and brain-penetrant GPR88 agonist, with an EC₅₀ of 25 nM in GPR88 cAMP functional assay. RTI-13951-33 hydrochloride reduces alcohol reinforcement and intake behaviors in rats.

For research use only. We do not sell to patients.

RTI-13951-33 hydrochloride Chemical Structure

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 644	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 644	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2750	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of RTI-13951-33 hydrochloride:

RTI-13951-33 Get quote

1 Publications Citing Use of MCE RTI-13951-33 hydrochloride

•bioRxiv. 2020 May.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

EC50: 25 nM (GPR88)^[1]

In Vitro

RTI-13951-33 is a potent, selective, and brain-penetrant GPR88 agonist, with an EC₅₀ of 25 nM in GPR88 cAMP functional assay. RTI-13951-33 elevates [³⁵S]-GTPγS binding (EC₅₀, 535 nM) in mouse striatal membranes but not in membranes from GPR88 KO mice^[1].
RTI-13951-33 has weak affinities at kappa opioid receptor (KOR; K_i, 2.29 μM), vesicular monoamine transporter (VMAT; K_i, 4.23 μM), and moderate affinity at serotonin transporter (SERT; K_i, 0.75 μM), however, RTI-13951-33 poorly inhibits SERT (IC₅₀, 25.1±2.7 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RTI-13951-33 (10 and 20 mg/kg, i.p.) dose-dependently decreases alcohol lever responses in a rat model of alcohol self-administration^[1].
RTI-13951-33 (10 mg/kg, i.p.) has sufficient brain penetration, with t_1/2 of 48 min and 87 min in rat plasma and brain^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

532.50

Formula

C₂₈H₃₅Cl₂N₃O₃

Appearance

Solid

Color

White to light yellow

SMILES

COCC1=CC=C(C2=CC=C(N(C[C@@H](N)[C@@H](C)OC)C([C@H]3[C@H](C4=CC=CC=N4)C3)=O)C=C2)C=C1.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (93.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (93.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8779 mL	9.3897 mL	18.7793 mL
5 mM	0.3756 mL	1.8779 mL	3.7559 mL
10 mM	0.1878 mL	0.9390 mL	1.8779 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (187.79 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.56%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (250 KB) HNMR (217 KB) LCMS (125 KB)

Handling Instructions (2659 KB)

References

[1]. Jin C, et al. Discovery of a Potent, Selective, and Brain-Penetrant Small Molecule that Activates the Orphan Receptor GPR88 and Reduces Alcohol Intake. J Med Chem. 2018 Aug 9;61(15):6748-6758. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.8779 mL	9.3897 mL	18.7793 mL	46.9484 mL
	5 mM	0.3756 mL	1.8779 mL	3.7559 mL	9.3897 mL
	10 mM	0.1878 mL	0.9390 mL	1.8779 mL	4.6948 mL
	15 mM	0.1252 mL	0.6260 mL	1.2520 mL	3.1299 mL
	20 mM	0.0939 mL	0.4695 mL	0.9390 mL	2.3474 mL
	25 mM	0.0751 mL	0.3756 mL	0.7512 mL	1.8779 mL
	30 mM	0.0626 mL	0.3130 mL	0.6260 mL	1.5649 mL
	40 mM	0.0469 mL	0.2347 mL	0.4695 mL	1.1737 mL
	50 mM	0.0376 mL	0.1878 mL	0.3756 mL	0.9390 mL
	60 mM	0.0313 mL	0.1565 mL	0.3130 mL	0.7825 mL
	80 mM	0.0235 mL	0.1174 mL	0.2347 mL	0.5869 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.