Tiopronin
Based on 1 publication(s) in Google Scholar
Tiopronin is a diffusible antioxidant, an antidote to heavy metal poisoning and a radioprotective agent. Tiopronin can control the rate of cystine precipitation and excretion and has the potential for cystinuria, rheumatoid arthritis and hepatic disorders.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 1953-02-2
- Formula: C5H9NO3S
- Molecular Weight:163.20
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Tiopronin
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
6.9 mM
Compound: 1, tiopronin
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Cytotoxicity against CHO cells incubated for 72 hrs by MTT assay
Cytotoxicity against CHO cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| HEK293 | IC50 |
0.15 mM
Compound: 1, tiopronin
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Cytotoxicity against human HEK293 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human HEK293 cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| HEK293 | IC50 |
0.26 mM
Compound: 1, tiopronin
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Cytotoxicity against drug resistant human HEK293 cells expressing MRP1 incubated for 72 hrs by MTT assay
Cytotoxicity against drug resistant human HEK293 cells expressing MRP1 incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| HeLa | IC50 |
5.14 mM
Compound: 1, tiopronin
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Cytotoxicity against multi drug-resistant human HeLa Tet-off cells expressing P-gp incubated for 72 hrs by MTT assay
Cytotoxicity against multi drug-resistant human HeLa Tet-off cells expressing P-gp incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| HeLa | IC50 |
6.43 mM
Compound: 1, tiopronin
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Cytotoxicity against human HeLa Tet-off cells incubated for 72 hrs by MTT assay
Cytotoxicity against human HeLa Tet-off cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| KB 3-1 | IC50 |
7.54 mM
Compound: 1, tiopronin
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Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| KB-V1 | IC50 |
0.147 mM
Compound: 1, tiopronin
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Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| MCF7 | IC50 |
12.27 mM
Compound: 1, tiopronin
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Cytotoxicity against human MCF7 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| MCF7-VP | IC50 |
0.29 mM
Compound: 1, tiopronin
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Cytotoxicity against drug-resistant human MCF7/VP16 cells expressing MRP1 incubated for 72 hrs by MTT assay
Cytotoxicity against drug-resistant human MCF7/VP16 cells expressing MRP1 incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| NCI/ADR-RES | IC50 |
9.05 mM
Compound: 1, tiopronin
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Cytotoxicity against drug-resistant human NCI-ADR-RES cells expressing P-gp incubated for 72 hrs by MTT assay
Cytotoxicity against drug-resistant human NCI-ADR-RES cells expressing P-gp incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| NCI-H460 | IC50 |
5.34 mM
Compound: 1, tiopronin
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Cytotoxicity against human H460 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human H460 cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| NIH3T3 | IC50 |
9.2 mM
Compound: 1, tiopronin
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Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| OVCAR-8 | IC50 |
5.99 mM
Compound: 1, tiopronin
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Cytotoxicity against human OVCAR8 Tet-off cells incubated for 72 hrs by MTT assay
Cytotoxicity against human OVCAR8 Tet-off cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
In in vitro Von Hippel-Lindau protein binding assay, the inhibitory effect of Tiopronin (NMPG) on HPH-2 was attenuated by escalating dose of ascorbate but not 2-ketoglutarate, cofactors of the enzyme[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1953-02-2
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Appearance Solid
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Molecular Weight 163.20
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Formula C5H9NO3S
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Color White to off-white
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SMILES
O=C(O)CNC(C(S)C)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Metabolites
Silybin Meglumine Mitigates CCl4-Induced Liver Fibrosis and Bile Acid Metabolism Alterations. [Abstract]2024 Oct 17;14(10):556. PMID: 39452937
Solvent & Solubility
DMSO : ≥ 100 mg/mL (612.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (612.75 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 130 mg/mL (796.57 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Pu Zhang, et al. Detection of tiopronin in body fluids and pharmaceutical products using red-emissive DNA-stabilized silver nanoclusters as a fluorescent probe. Mikrochim Acta. 2019 Aug 8;186(9):609. [Content Brief]
[2]. Soohwan Yum, et al. N-(2-Mercaptopropionyl)-glycine, a diffusible antioxidant, activates HIF-1 by inhibiting HIF prolyl hydroxylase-2: implication in amelioration of rat colitis by the antioxidant. Biochem Biophys Res Commun. 2014 Jan 17;443(3):1008-13. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 6.1275 mL | 30.6373 mL | 61.2745 mL | 153.1863 mL |
| 5 mM | 1.2255 mL | 6.1275 mL | 12.2549 mL | 30.6373 mL | |
| 10 mM | 0.6127 mL | 3.0637 mL | 6.1275 mL | 15.3186 mL | |
| 15 mM | 0.4085 mL | 2.0425 mL | 4.0850 mL | 10.2124 mL | |
| 20 mM | 0.3064 mL | 1.5319 mL | 3.0637 mL | 7.6593 mL | |
| 25 mM | 0.2451 mL | 1.2255 mL | 2.4510 mL | 6.1275 mL | |
| 30 mM | 0.2042 mL | 1.0212 mL | 2.0425 mL | 5.1062 mL | |
| 40 mM | 0.1532 mL | 0.7659 mL | 1.5319 mL | 3.8297 mL | |
| 50 mM | 0.1225 mL | 0.6127 mL | 1.2255 mL | 3.0637 mL | |
| 60 mM | 0.1021 mL | 0.5106 mL | 1.0212 mL | 2.5531 mL | |
| 80 mM | 0.0766 mL | 0.3830 mL | 0.7659 mL | 1.9148 mL | |
| 100 mM | 0.0613 mL | 0.3064 mL | 0.6127 mL | 1.5319 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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