TBX5 Antibody
(Synonyms: HOS; TBX5)TBX5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TBX5.
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Host:
Rabbit
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Isotype:
IgG
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Application:
IHC-P, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:50-100 | 1:1000-3000 |
Product Details
TBX5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TBX5.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 58 kDa
Purified recombinant fragment of TBX5.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TBX5 is a DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus; Cytoplasm
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Isoforms & Post-Translational Modification
TBX5 has 3 isoforms, Q99593-1: amino acid length is 518, molecular weight is 57711 Da (predicted); Q99593-2: amino acid length is 349, molecular weight is 39196 Da (predicted); Q99593-3: amino acid length is 468, molecular weight is 52659 Da (predicted).
Acetylation at Lys-339 by KAT2A and KAT2B promotes nuclear retention -
Subunit
Monomer.
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SwissProt ID
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Synonyms
HOS; TBX5
Documentation
[1]. Zhang XL, et al. TBX5 loss-of-function mutation contributes to familial dilated cardiomyopathy. Biochem Biophys Res Commun. 2015 Mar 27;459(1):166-71. [Content Brief]
[2]. Zhou W, et al. A novel TBX5 loss-of-function mutation associated with sporadic dilated cardiomyopathy. Int J Mol Med. 2015 Jul;36(1):282-8. [Content Brief]
[3]. Wang ZC, et al. Prevalence and Spectrum of TBX5 Mutation in Patients with Lone Atrial Fibrillation. Int J Med Sci. 2016;13(1):60-7. [Content Brief]
[4]. Guo DF, et al. TBX5 loss-of-function mutation contributes to atrial fibrillation and atypical Holt-Oram syndrome. Mol Med Rep. 2016 May;13(5):4349-56. [Content Brief]
[5]. Ghosh TK, et al. Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development. J Mol Cell Cardiol. 2018 Jan;114:185-198. [Content Brief]
[6]. Li QY, et al. Holt-Oram syndrome is caused by mutations in TBX5, a member of the Brachyury (T) gene family. Nat Genet. 1997 Jan;15(1):21-9. [Content Brief]
[7]. Pradhan L, et al. Intermolecular Interactions of Cardiac Transcription Factors NKX2.5 and TBX5. Biochemistry. 2016 Mar 29;55(12):1702-10. [Content Brief]
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