Apoptosis inducer 20

Cat. No.: HY-161814: Data Sheet Handling Instructions
FAQs
Technical Support

Apoptosis inducer 20 (12) causes G2/M cell cycle arrest and induction of apoptosis via caspase 3/7 activation, which occurred during M arrest. Apoptosis inducer 20 is a novel indolic benzenesulfonamide with anti-proliferative effect against a broad panel of cancer cell lines, which is proming for research of new anti-mitotic agents.

For research use only. We do not sell to patients.

Apoptosis inducer 20 Chemical Structure

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	22.1 nM Compound: 12	Antiproliferative activity against human A-375 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human A-375 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
BT-474	IC₅₀	68.3 nM Compound: 12	Antiproliferative activity against human BT-474 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human BT-474 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
CAKI-1	IC₅₀	56.2 nM Compound: 12	Antiproliferative activity against human CAKI-1 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human CAKI-1 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
HEK293	IC₅₀	23.4 nM Compound: 12	Antiproliferative activity against human HEK293 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human HEK293 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
HT-1080	IC₅₀	9.9 nM Compound: 12	Antiproliferative activity against human HT-1080 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human HT-1080 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
HeLa	IC₅₀	1.7 nM Compound: 12	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 38959729]
HeLa	IC₅₀	11 nM Compound: 12	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
HeLa	IC₅₀	16.3 nM Compound: 12	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs in presence of verapamil by MTT assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs in presence of verapamil by MTT assay	[PMID: 38959729]
HeLa	IC₅₀	22.3 nM Compound: 12	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs in absence of verapamil by MTT assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs in absence of verapamil by MTT assay	[PMID: 38959729]
MCF7	IC₅₀	13.7 nM Compound: 12	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
MDA-MB-468	IC₅₀	13 nM Compound: 12	Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
MES-SA	IC₅₀	8.9 nM Compound: 12	Antiproliferative activity against human MES-SA cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human MES-SA cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]
TERT-RPE1	IC₅₀	80.4 nM Compound: 12	Antiproliferative activity against human RPE-1 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay Antiproliferative activity against human RPE-1 cells assessed as reduction in cell viability incubated for 72 hrs by CelltiterGlo luminescent assay	[PMID: 38959729]

In Vitro

Apoptosis inducer 20 inhibits cell growth activity in different cancer cell lines. The melanoma cell-line MelMet5 is the most sensitive cell-line, followed by HeLa^[1].
Apoptosis inducer 20 (100 nM, 24 h) causes G2/M cell cycle arrest and induction of apoptosis via caspase 3/7 activation in MES-SA cells^[1].

Antiproliferative IC50 values of active sulfonamides on HeLa, MCF7, BT-474, MDA-MB468, MES-SA, HT-1080, MelMet5, A375, WM-1366, and Caki1 cancer cell lines^[1]

	HeLa	MCF7	BT-474	MDA-MB468	MES-SA	HT-1080	MelMet5	A375	WM-1366	Caki1
12	11.0	13.7	68.3	13.0	8.9	9.9	8.9	22.1	52.8	56.2

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	MES-SA cells (human uterine sarcoma)
Concentration:	100 nM
Incubation Time:	24 h
Result:	Increased the percentage of cells in the G2/M ensemble from 13.3 % to 83.6-89.7 % at the expense of the S (from 27 % to 6-9 %) and G1 phases (from 60 % to 4-7 %) in MES-SA cells.

Apoptosis Analysis^[1]

Cell Line:	MES-SA cells (human uterine sarcoma)
Concentration:	100 nM
Incubation Time:	3 days
Result:	Induced caspase activation and therefore cell apoptosis in a time-dependent manner in MES-SA cells.

Immunofluorescence^[1]

Cell Line:	MES-SA cells (human uterine sarcoma)
Concentration:	10 nM
Incubation Time:	24 h
Result:	Induced microtubule nonfunction, depolymerization and the cell loseed its characteristic shape in MES-SA cells.

Molecular Weight

415.46

Formula

C₂₀H₂₁N₃O₅S

SMILES

CN1C=CC2=C1C=CC(N(CC#N)S(C3=CC(OC)=C(OC)C(OC)=C3)(=O)=O)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Fuentes-Martín R, et al. Promising anti-proliferative indolic benzenesulfonamides alter mechanisms with sulfonamide nitrogen substituents[J]. Eur J Med Chem. 2024 Sep 5;275:116617. [Content Brief]