ARP  (Synonyms: Aldehyde reactive probe)

ARP (Aldehyde reactive probe) is an aldehyde-reactive probe. ARP specifically labels AP sites in DNA with biotin residues. ARP detects abasic sites induced by RNA oxidation. ARP is suitable for studies involving the quantification of AP sites, or the detection of other aldehyde-containing DNA damages and abasic sites induced by RNA oxidation^[1][2].

ARP (100 μL; 1 h at 37 °C) detects AP sites and related aldehyde-containing damages in DNA isolated from heat-inactivated, X-irradiated E. coli AB1157 cells, with signal intensity proportional to radiation dose^[1].
ARP (100 μL; 1 h at 37 °C) detects AP sites formed in the DNA of MMS-treated E. coli AB1157 cells. Its signal is proportional to the concentration of MMS and decreases during the repair process after treatment^[1].
ARP (2 mM; 1 h) specifically reacts with apurinic sites in acid-apurinized yeast tRNA^Phe, and its reaction activity is directly proportional to the degree of apurinization^[2].
ARP (0-10 mM; 1 h) specifically binds to abasic sites in chemically synthesized RNA, with a limit of detection of 10 fmoles of abasic sites^[2].
The reactivity of ARP (0-10 mM; 0.5-2 h) with synthetic abasic RNA increases with elevated temperature, prolonged incubation time up to 1 h, acidic pH, and increased ARP concentration^[2].
ARP (2 mM; 1 h) detects apurinic/apyrimidinic sites in in vitro synthesized RNA irradiated with γ-rays in a dose-dependent manner, and this effect occurs at doses ≥5 Gy^[2].
ARP (2 mM; 1 h) detects apurinic/apyrimidinic (AP) sites in in vitro synthesized RNA oxidized by the Fenton reaction in a dose-dependent manner, and covalently binds to abasic glycosyl groups in Fenton reaction-oxidized RNA to form ribose-ARP adducts detectable by LC/MS^[2].
ARP (2 mM; 1 h) detects apurinic/apyrimidinic sites in in vitro synthesized RNA oxidized by peroxynitrite in a dose-dependent manner^[2].
ARP (2 mM; 1 h) detected an increase in the formation of abasic sites in the total RNA of HeLa cells treated with hydrogen peroxide or peroxynitrite^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

331.39

C₁₂H₂₁N₅O₄S

139585-03-8

Solid

NOCC(NNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O

Room temperature in continental US; may vary elsewhere.

• [1]. Kubo K, et al. A novel, sensitive, and specific assay for abasic sites, the most commonly produced DNA lesion. Biochemistry. 1992;31(14):3703-3708.  [Content Brief]

  [2]. Tanaka M, et al. An assay for RNA oxidation induced abasic sites using the Aldehyde Reactive Probe. Free Radic Res. 2011;45(2):237-247.  [Content Brief]