Autophagy activator-2

Autophagy activator-2 (compound 2) induces autophagosome formation in eGFP-LC3 HeLa cells with an EC₅₀ of 14.33 μM, acting as an autophagy activator near its EC₅₀ and a late-stage inhibitor at higher concentrations^[1].
Autophagy activator-2 (20, 40 μM; 6 h in HeLa cells; 20 μM; 24 h in fibroblasts) slightly reduces lysosomal proteolytic activity in wild-type HeLa cells at 20 and 40 μM but significantly enhances proteolytic activity in NPC1^I1061T fibroblasts at 20 μM^[1].
Autophagy activator-2 (10-80 μM; 24 h) is noncytotoxic to NPC1 fibroblasts^[1].
Autophagy activator-2 (80 μM; 24 h) does not inhibit mTOR kinase activity in NPC1^I1061T fibroblast^[1].
Autophagy activator-2 (80 μM; 24 h) reduces expression of multiple lysosomal hydrolases and increases glycosylated LAMP1 levels in NPC1^I1061T fibroblasts^[1].
Autophagy activator-2 (80 μM; 24 h) does not reduce unesterified cholesterol accumulation in NPC1 null HeLa cells, demonstrating its activity is dependent on NPC1 expression^[1].
Autophagy activator-2 (10, 20 μM; 24 h) increases Endo H-sensitive NPC1 protein levels in fibroblasts, without affecting wild-type NPC1 expression^[1].
Autophagy activator-2 (40, 80 μM; 24 h) does not broadly suppress proteasomal or autophagic degradation or alter ATF4 expression in I1061T NPC1 mutant fibroblasts, while elevating the LC3-II/LC3-I ratio and showing no enhanced ER stress^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.