Acute hypoxia promotes the liver angiogenesis of largemouth bass (Micropterus salmoides) by HIF - Dependent pathway

  • Fish Shellfish Immunol. 2022 Dec:131:264-273. doi: 10.1016/j.fsi.2022.08.007.
Liulan Zhao  1 ,  Xiaohong Tang  2 ,  Rui Huang  3 ,  Qiao Liu  4 ,  Lei Liao  5 ,  Yifan Hu  6 ,  Kuo He  7 ,  Xin Zhang  8 ,  Jiazhong Guo  9 ,  Shiyi Chen  10 ,  Song Yang  11
Affiliations
  • 1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 2. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Fish Resources and Environment in the Lpper Reaches of the Yangtze River Observation and Research Station of Sichuan Province, Chengdu, Sichuan, 610011, China. Electronic address: [email protected].
  • 3. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 4. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 5. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 6. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 7. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 8. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 9. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 10. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
  • 11. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: [email protected].
PMID: 35940542 DOI: 10.1016/j.fsi.2022.08.007
Abstract

A 24-h hypoxia exposure experiment was conducted to determine how hypoxia exposure induce liver angiogenesis in largemouth bass. Nitrogen (N2) was pumped into water to exclude dissolved oxygen into 1.2 ± 0.2 mg/L, and liver tissues were sampled during hypoxia exposure of 0 h, 4 h, 8 h, 12 h, 24 h and re-oxygenation for 12 h. Firstly, the results showed that hypoxia exposure promoted the angiogenesis occurrence by immunohistochemical analysis of vascular endothelial growth factor receptor 2 (VEGFR2). Secondly, the concentration of vasodilation factor increased and it's activity was elevated during 8 h exposure, such as nitric oxide (NO) and nitric oxide synthase (NOS) (p < 0.05). Thirdly, hypoxia exposure promoted angiogenesis through up-regulation the expression of matrix metalloproteinase 2 (MMP-2), jagged, protein kinase B (Akt), phosphoinositide-3-kinase (PI3K), mitogen-activated protein kinase (MAPK) at 4 h; contrarily, the expression of inhibiting angiogenesis genes presented up-regulated at 8 h (p < 0.05), such as matrix metalloproteinase inhibitor-2 (TIMP-2), matrix metalloproteinase inhibitor-3 (TIMP-3). Finally, the genes and proteins that regulate angiogenesis presented obvious chronological order. Parts of them promoted the budding and extension of blood vessels were up-regulated during 4 h-8 h (p < 0.05), such as vascular endothelial growth factor a (VEGFA), VEGFR2, monocarboxylic acid transporter 1 (MCT1), CD147, prolyl hydroxylase (PHD), nuclear factor kappa-B (NF-κB); Other part of them promoted blood vessel maturation were highly expressed during 12 h-24 h (p < 0.05), such as angiogenin-1 (ANG-1) and angiogenin-2 (ANG-2). In short, acute hypoxia can promote the liver angiogenesis of largemouth bass by HIF - dependent pathway.

Keywords
Angiogenesis; Hypoxia; Hypoxia inducible factor; Micropterus salmoides; Vascular endothelial growth factor.
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