Costunolide
Based on 6 publication(s) in Google Scholar
Costunolide ((+)-Costunolide) is a naturally occurring sesquiterpene lactone, with antioxidative, anti-inflammatory, antiallergic, bone remodeling, neuroprotective, hair growth promoting, anticancer, and antidiabetic properties. Costunolide can induce cell cycle arrest and apoptosis on breast cancer cells.
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- Pureté: 99.92%
- CAS No.: 553-21-9
- Formule: C15H20O2
- Masse moléculaire:232.32
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Stockage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
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Publications Citing Use of MedChemExpress (MCE) Costunolide
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Cell Proliferation/Viability Assay
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RT-PCR
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Bio/Physico-chemical Assay
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RT-PCR
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Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
12.3 μM
Compound: 1
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Cytotoxicity against human A549 cell line
Cytotoxicity against human A549 cell line
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[PMID: 16766184] |
| C6 | IC50 |
4.1 μM
Compound: 1
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Cytotoxicity against rat C6 cells
Cytotoxicity against rat C6 cells
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[PMID: 26226279] |
| DU-145 | IC50 |
29.2 μM
Compound: 1
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Cytotoxicity against human DU145 cell line
Cytotoxicity against human DU145 cell line
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[PMID: 16766184] |
| HBL-100 | IC50 |
10.3 μM
Compound: 1
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Cytotoxicity against human HBL100 cell line
Cytotoxicity against human HBL100 cell line
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[PMID: 16766184] |
| HL-60 | IC50 |
2.1 μM
Compound: 1
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Cytotoxicity against human HL60 cells
Cytotoxicity against human HL60 cells
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[PMID: 26226279] |
| HL-60 | IC50 |
7.7 μM
Compound: 2
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Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry
Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry
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[PMID: 22985027] |
| K562 | IC50 |
14.5 μM
Compound: 1
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Cytotoxicity against human K562 cell line
Cytotoxicity against human K562 cell line
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[PMID: 16766184] |
| KB | ED50 |
0.57 μg/mL
Compound: 4, Costunolide
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 17336532] |
| KB | ED50 |
2.46 μM
Compound: costunolide
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 18329753] |
| KB | IC50 |
28.9 μM
Compound: 1
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Cytotoxicity against human KB cell line
Cytotoxicity against human KB cell line
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[PMID: 16766184] |
| KG-1a | IC50 |
4.1 μM
Compound: 1
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Cytotoxicity against human KG1a cells
Cytotoxicity against human KG1a cells
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[PMID: 26226279] |
| Macrophage | IC50 |
1.2 μg/mL
Compound: 9
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Antiinflammatory activity in mouse macrophages assessed as LPS-induced nitric oxide production
Antiinflammatory activity in mouse macrophages assessed as LPS-induced nitric oxide production
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10.1039/C2MD20172K |
| MIA PaCa-2 | IC50 |
17.7 μM
Compound: 1
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Cytotoxicity against human MiaPaca2 cell line
Cytotoxicity against human MiaPaca2 cell line
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[PMID: 16766184] |
| NALM-6 | IC50 |
65 μM
Compound: Costunolide
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Inhibition of telomerase in human NALM6 cells by TRAP assay
Inhibition of telomerase in human NALM6 cells by TRAP assay
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10.1039/C0MD00241K |
| NIH3T3 | IC50 |
13.9 μM
Compound: 1
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Cytotoxicity against mouse NIH3T3 cell line
Cytotoxicity against mouse NIH3T3 cell line
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[PMID: 16766184] |
| PA-1 | IC50 |
10.6 μM
Compound: 1
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Cytotoxicity against human PA1 cell line
Cytotoxicity against human PA1 cell line
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[PMID: 16766184] |
| RAW264.7 | IC50 |
>50 μM
Compound: 22
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Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
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[PMID: 33939429] |
| SW-620 | IC50 |
7.8 μM
Compound: 1
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Cytotoxicity against human SW620 cell line
Cytotoxicity against human SW620 cell line
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[PMID: 16766184] |
| T-cell | IC50 |
2.57 μM
Compound: Cos
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Immunomodulatory activity in C57BL/6 mouse mesenteric lymph node T cells assessed as inhibition of concanavalin A stimulated T-cell proliferation incubated for 68 hrs by CCK-8 assay
Immunomodulatory activity in C57BL/6 mouse mesenteric lymph node T cells assessed as inhibition of concanavalin A stimulated T-cell proliferation incubated for 68 hrs by CCK-8 assay
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[PMID: 37017305] |
Costunolide inhibits the colony formation, migrative and invasive abilities of the H1299 cells in a dose or time dependent manner[2].
Costunolide (6.7-215.2 μM; 24 hours) inhibits the viability of H1299 cells in a dose-dependent manner, with an IC50 of 23.93 μM[2].
Costunolide (12.0-48.0 μM; 48 hours) induces apoptosis in H1299 cells[2].
Costunolide (12-48.0 μM; 6-12 hours) regulates metastasis- and proliferation-associated mRNA expression[2].
Costunolide regulates epithelial-to-mesenchymal transition (EMT)-associated protein expression[2].
Costunolide regulates c-Myc mediated apoptosis signaling and 14-3-3-mediated signaling pathways in breast cancer cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H1299 cells
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Concentration:6.7 μM, 13.5 μM, 26.9 μM, 107.6 μM, 215.2 μM
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Incubation Time:24 hours
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Result:Inhibited the viability of H1299 cells (MTT assay).
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Cell Line:H1299 cells
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Concentration:0 µM, 12.0 µM, 24.0 µM, 48.0 µM
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Incubation Time:48 hours
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Result:Significantly promoted apoptosis at 24.0 µM and 48.0 µM.
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Cell Line:H1299 cells
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Concentration:0 µM, 12.0 µM, 24.0 µM, 48.0 µM
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Incubation Time:6 hours, 12 hours
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Result:Regulated the metastasis- and proliferation-associated mRNA levels in a dose-dependent manner.
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Cell Line:H1299 cells
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Concentration:0 µM, 12.0 µM, 24.0 µM, 48.0 µM
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Incubation Time:48 hours
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Result:Significantly inhibited the EMT of H1299 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:4 weeks old female BALB/c nude mice, MDA-MB-231 cells xenograft mouse models[3]
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Dosage:20 mg/kg
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Administration:Intraperitoneal injection, daily, for 30 days
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Result:Reduced the expression levels of c-Myc and p-AKT and elevated the expression levels of p53 and p-14-3-3.
Chemical Information
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CAS No. 553-21-9
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Appearance Solid
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Masse moléculaire 232.32
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Formule C15H20O2
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Color White to yellow
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SMILES
O=C(O[C@@]1([H])[C@@]2([H])CC/C(C)=C/CC/C(C)=C/1)C2=C
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Synonyms
(+)-Costunolide; Costus lactone
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Structure Classification
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell Mol Biol Lett
Costunolide reduces glycolysis-associated activation of hepatic stellate cells via inhibition of hexokinase-2. [Abstract]2019 Aug 14:24:52. PMID: 31428167
Costunolide purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2019 Aug 14:24:52. [Abstract]
Costunolide reduces HSC activation. Primary rat HSCs at passages 3 through 5 were treated with Costunolide (0-30 μM) at the indicated concentrations for 24 h. MTT assay for evaluating cell viability, which is presented as a percentage of the control.
Costunolide purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2019 Aug 14:24:52. [Abstract]
Costunolide reduces HSC activation. Primary rat HSCs at passages 3 through 5 were treated with Costunolide (0-30 μM) at the indicated concentrations for 24 h. Real-time PCR for determining the mRNA expression of α-SMA and collagen I.
Costunolide purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2019 Aug 14:24:52. [Abstract]
Costunolide reduces HSC activation. Primary rat HSCs at passages 3 through 5 were treated with Costunolide (0-30 μM) at the indicated concentrations for 24 h. Western blot assay for determining the protein expression of α-SMA and collagen I with quantification of the blots.
Costunolide purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2019 Aug 14:24:52. [Abstract]
Costunolide blocks aerobic glycolysis in HSCs. Primary rat HSCs at passages 3 through 5 were treated with Costunolide (0-30 μM) at the indicated concentrations for 24 h. Measurement of glucose uptake using absorbance at 570/610 nm.
Costunolide purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2019 Aug 14:24:52. [Abstract]
Blockade of aerobic glycolysis contributes to the reduction in HSC activation by costunolide. Primary rat HSCs at passages 3 through 5 were treated with Costunolide (30 μM) and/or 2-DG at the indicated concentrations for 24 h. Real-time PCR for determining the mRNA expression of α-SMA and collagen I.
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Phytomedicine
Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]2025 May:140:156484. PMID: 40090046 -
Molecules
Costunolide Plays an Anti-Neuroinflammation Role in Lipopolysaccharide-Induced BV2 Microglial Activation by Targeting Cyclin-Dependent Kinase 2. [Abstract]2020 Jun 19;25(12):2840. PMID: 32575562 -
ACS Omega
Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer. [Abstract]2024 Feb 28;9(10):11870-11882. PMID: 38496939 -
Gene
Activation of p53 by costunolide blocks glutaminolysis and inhibits proliferation in human colorectal cancer cells. [Abstract]2018 Dec 15:678:261-269. PMID: 30103008
Costunolide purchased from MedChemExpress. Usage Cited in: Gene. 2018 Dec 15:678:261-269. [Abstract]
Western blot analysis of mTOR phosphorylation at Ser2448 and Ser2481 with quantification.
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Solvant et solubilité
DMSO : 100 mg/mL (430.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 25 mg/mL (107.61 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (287 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Dae Yong Kim, et al. Costunolide-A Bioactive Sesquiterpene Lactone with Diverse Therapeutic Potential. Int J Mol Sci. 2019 Jun; 20(12): 2926. [Content Brief]
[2]. Minyan Wei, et al. Costunolide induces apoptosis and inhibits migration and invasion in H1299 lung cancer cells. Oncol Rep. 2020 Jun;43(6):1986-1994. [Content Brief]
[3]. Zhangxiao Peng, et al. Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis through c-Myc/p53 and AKT/14-3-3 pathway.Sci Rep. 2017; 7: 41254. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 4.3044 mL | 21.5220 mL | 43.0441 mL | 107.6102 mL |
| 5 mM | 0.8609 mL | 4.3044 mL | 8.6088 mL | 21.5220 mL | |
| 10 mM | 0.4304 mL | 2.1522 mL | 4.3044 mL | 10.7610 mL | |
| 15 mM | 0.2870 mL | 1.4348 mL | 2.8696 mL | 7.1740 mL | |
| 20 mM | 0.2152 mL | 1.0761 mL | 2.1522 mL | 5.3805 mL | |
| 25 mM | 0.1722 mL | 0.8609 mL | 1.7218 mL | 4.3044 mL | |
| 30 mM | 0.1435 mL | 0.7174 mL | 1.4348 mL | 3.5870 mL | |
| 40 mM | 0.1076 mL | 0.5381 mL | 1.0761 mL | 2.6903 mL | |
| 50 mM | 0.0861 mL | 0.4304 mL | 0.8609 mL | 2.1522 mL | |
| 60 mM | 0.0717 mL | 0.3587 mL | 0.7174 mL | 1.7935 mL | |
| 80 mM | 0.0538 mL | 0.2690 mL | 0.5381 mL | 1.3451 mL | |
| 100 mM | 0.0430 mL | 0.2152 mL | 0.4304 mL | 1.0761 mL |
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