Cat. No.: HY-15680

O-Propargyl-Puromycin

33 Cited Publications

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Based on 33 publication(s) in Google Scholar

O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor. O-Propargyl-Puromycin is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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Pureté: 99.71%
CAS No.: 1416561-90-4
Formule: C₂₄H₂₉N₇O₅
Masse moléculaire:495.53

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

O-Propargyl-Puromycin Chemical Structure

CAS No.: 1416561-90-4

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Activité biologique
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (33)
Solvant et solubilité
- In Vitro
- In Vivo
Protocole
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin. O-propargyl-puromycin forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tissues from uninjected mice shows low nonspecific staining, whereas tissues from O-propargyl-puromycin-injected mice display specific patterns of O-propargyl-puromycin incorporation into nascent proteins. In the small intestine, translation is strongest in cells in the crypts and at the base of intestinal villi, consistent with the high proliferative and secretory activity of these cells. The stain is particularly strong in Paneth cells, which are located close to the base of the crypts and are filled with secretory vesicles. The intense O-propargyl-puromycin labeling of vesicles in Paneth cells suggests that prematurely terminated, O-propargyl-puromycin-conjugated secretory proteins are translocated into the endoplasmic reticulum (ER) lumen^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1416561-90-4
Appearance Solid
Masse moléculaire 495.53
Formule C₂₄H₂₉N₇O₅
Color White to off-white
SMILES

CN(C)C1=C2C(N([C@H]3[C@H](O)[C@H](NC([C@@H](N)CC4=CC=C(OCC#C)C=C4)=O)[C@@H](CO)O3)C=N2)=NC=N1
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (33)

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Nature

A non-canonical tricarboxylic acid cycle underlies cellular identity. [Abstract]

2022 Mar;603(7901):477-481. PMID: 35264789
Cell

Persistent and transient senescent cells contribute to brain-barrier development. [Abstract]

2026 Jun 10:S0092-8674(26)00581-7. PMID: 42269604
Cell

Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle. [Abstract]

2026 Apr 30;189(9):2684-2699.e21. PMID: 41763199
Cell Metab

OGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesis. [Abstract]

2025 Sep 16:S1550-4131(25)00381-X. PMID: 40961937

O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Sep 16:S1550-4131(25)00381-X. [Abstract]

Mice were injected with MLL-AF9 AML, switched to a BCAA-free diet and treated with 5 + 3. At the point of maximum response, OPP was administered by i.p. injection (50 mg/kg) and the mouse was sacrificed after 1 h. BM cells were isolated, fixed, and permeabilized before an azide-alkyne iClick-iT reaction was performed to fluorescently label nascent peptides with OPP. OPP incorporation was measured by flow cytometry.
Cell Metab

Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness. [Abstract]

2023 Jun 6;35(6):1009-1021.e9. PMID: 37084733
Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. [Abstract]

2021 Jul 1;28(7):1275-1290.e9. PMID: 33711283

O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2021 Jul 1;28(7):1275-1290.e9. [Abstract]

Global protein synthesis rates of various TF-1 cells stably expressing control shLuc, single or double knockdown of HECTD1 and ZNF622 were measured using OP-Puro assay. OP-Puro was added to cell culture at the final concentration of 20uM for 1 hour at a 37°C incubator.

O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2021 Jul 1;28(7):1275-1290.e9. [Abstract]

Representative histogram plot of protein synthesis rate in BM HSCs of 5-FU challenged mice as determined by in vivo OP-Puro assay. To detect protein synthesis rate in vivo, primary or stressed mice were injected intraperitoneally with OP-Puro (50mg/kg, pH6.4–6.6 in PBS) for 1 hour before euthanasia.
Cancer Res

Ribonuclease 5/Angiogenin Suppresses Intestinal Tumor Initiation by Maintaining Crypt Homeostasis. [Abstract]

2026 Feb 24. PMID: 41734376
Nat Commun

WNK1 signalling regulates amino acid transport and mTORC1 activity to sustain acute myeloid leukaemia growth. [Abstract]

2025 May 27;16(1):4920. PMID: 40425534

O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 27;16(1):4920. [Abstract]

Protein synthesis rates as measured by incorporation of OP-puro in Wnk1fl/− MA9 leukaemia cells treated with 10 µM Compound 12 at the indicated time points. Cells were labelled with 10 µM OP-Puro for 30 min.
Sci Adv

Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress. [Abstract]

2026 May 22;12(21):eadz7896. PMID: 42172329
Sci Adv

The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles. [Abstract]

2025 Sep 12;11(37):eadx4511. PMID: 40938992
Sci Adv

N-acetyltransferase NAT10 controls cell fates via connecting mRNA cytidine acetylation to chromatin signaling. [Abstract]

2024 Jan 12;10(2):eadh9871. PMID: 38215194
Cancer Biol Med

SNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA. [Abstract]

2021 Mar 12;19(1):104-119. PMID: 33710804
J Exp Med

Mitochondria transfer mediates stress erythropoiesis by altering the bioenergetic profiles of early erythroblasts through CD47. [Abstract]

2022 Dec 5;219(12):e20220685. PMID: 36112140
J Exp Med

TREM2-dependent lipid droplet biogenesis in phagocytes is required for remyelination. [Abstract]

2021 Oct 4;218(10):e20210227. PMID: 34424266
Cell Rep

A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential. [Abstract]

2025 Nov 25;44(11):116496. PMID: 41166305
Cell Rep

Defective ribosome assembly impairs leukemia progression in a murine model of acute myeloid leukemia. [Abstract]

2024 Oct 15;43(11):114864. PMID: 39412990
Mol Cell Proteomics

Proteogenomic Profiling Reveals Small ORFs and Functional Microproteins in Activated T Cells. [Abstract]

2025 Feb 4:100914. PMID: 39914663
Int J Mol Sci

A Coordinated Translational Control Mediated by eEF2 Phosphorylation Safeguards Erythroid Differentiation. [Abstract]

2025 May 16;26(10):4801. PMID: 40429942
Int Immunopharmacol

Targeting lipid peroxidation-associated ferroptosis suppresses lung carcinoma progression by regulating cell cycle arrest. [Abstract]

2024 Jun 24:138:112518. PMID: 38917528
STAR Protoc

Metabolic labeling and LC-MS/MS-based identification of interleukin-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum. [Abstract]

2023 Mar 31;4(2):102195. PMID: 37004159
STAR Protoc

Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro. [Abstract]

2020 Sep 18;1(2):100103. PMID: 33000005
bioRxiv

2026 Apr 18.
bioRxiv

2026 Mar 9.
bioRxiv

Differentiation stage-specific use of cap-independent and cap-dependent translation initiation in hematopoiesis. [Abstract]

2025 Sep 25:2025.09.24.678136. PMID: 41040404
bioRxiv

2025 March 17.
Patent

US20240392239A1.
Patent

US20240301413A1.
Research Square Preprint

2023 Oct 16.
bioRxiv

2023 Mar 5.
bioRxiv

2023 Jan 23.
bioRxiv

January 14, 2022.
Methods Mol Biol

Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer. [Abstract]

2021:2318:255-266. PMID: 34019295
Elife

Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma. [Abstract]

2015 Oct 24;4:e09436. PMID: 26499495

O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Elife. 2015 Oct 24;4:e09436. [Abstract]

Polypeptide synthetic activity is determined by OP-puromycin staining.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (201.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0180 mL

10.0902 mL

20.1804 mL

5 mM

0.4036 mL

2.0180 mL

4.0361 mL

10 mM

0.2018 mL

1.0090 mL

2.0180 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocole

Animal Administration^[1]

Mice: 100 μL of a 20 mM solution of O-propargyl-puromycin in PBS are injected intraperitoneally into a 3-wk-old mouse, and a mouse injected with 100 μL of PBS is used as negative control. Various organs are harvested after 1 h and are fixed in formalin overnight. Organ fragments are embedded in paraffin, sectioned, and ished with xylene to remove the paraffin. After ishing with ethanol and rehydration in TBS, the tissue sections are stained with 20 μM tetramethylrhodamine(TMR)-azide. The tissue sections are counterstained with Hoechst, mounted in standard mounting media, and are then imaged by fluorescence microscopy and DIC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (276 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Liu J, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):413-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0180 mL	10.0902 mL	20.1804 mL	50.4510 mL
	5 mM	0.4036 mL	2.0180 mL	4.0361 mL	10.0902 mL
	10 mM	0.2018 mL	1.0090 mL	2.0180 mL	5.0451 mL
	15 mM	0.1345 mL	0.6727 mL	1.3454 mL	3.3634 mL
	20 mM	0.1009 mL	0.5045 mL	1.0090 mL	2.5226 mL
	25 mM	0.0807 mL	0.4036 mL	0.8072 mL	2.0180 mL
	30 mM	0.0673 mL	0.3363 mL	0.6727 mL	1.6817 mL
	40 mM	0.0505 mL	0.2523 mL	0.5045 mL	1.2613 mL
	50 mM	0.0404 mL	0.2018 mL	0.4036 mL	1.0090 mL
	60 mM	0.0336 mL	0.1682 mL	0.3363 mL	0.8409 mL
	80 mM	0.0252 mL	0.1261 mL	0.2523 mL	0.6306 mL
	100 mM	0.0202 mL	0.1009 mL	0.2018 mL	0.5045 mL

Keywords

O-Propargyl-Puromycin

33 Cited Publications

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O-Propargyl-Puromycin

Publications Citing Use of MedChemExpress (MCE) O-Propargyl-Puromycin

Activité biologique

Chemical Information

Publications (33)

Solvant et solubilité

Protocole

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

O-Propargyl-Puromycin Related Classifications

HY-15680 Related Classifications