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JunB Antibody (YA325)

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Based on 1 Customer Validation

JunB Antibody (YA325) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JunB.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • JunB Antibody (YA325) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

JunB Antibody (YA325) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JunB.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42/43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database

Entrez Gene: 3726 Human

SwissProt: P17275 Human

Immunogen

Synthetic peptide within N-terminal human JunB.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102345

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Livraison

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for JunB Antibody (YA325)
    Western blot analysis of extracts from MCF-7(lane 2(20μg) , SiHa(lane 3(20μg) ,U937(lane 4(20μg)and THP-1( lane 5(20μg) using JunB Antibody (HY-P80200). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunocytochemistry analysis of HeLa cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for JunB Antibody (YA325)
    Immunocytochemistry analysis of HepG2 cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity)

  • Subcellular Localization

    Nucleus

  • Expression


    Induction:By growth factors

  • Subunit

    Binds DNA as a homodimer or as a heterodimer with another member of the Jun/Fos family.

  • SwissProt ID

    P17275

  • Gene ID
    3726 [NCBI]
  • Research Field

    Epigenetics and Nuclear Signaling

JunB Antibody (YA325) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg) , SiHa(lane 3(20μg) ,U937(lane 4(20μg)and THP-1( lane 5(20μg) using JunB Antibody (HY-P80200). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • JunB Antibody (YA325) Product Image
    1/16
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • JunB Antibody (YA325) Product Image
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg) , SiHa(lane 3(20μg) ,U937(lane 4(20μg)and THP-1( lane 5(20μg) using JunB Antibody (HY-P80200). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for JunB Antibody (YA325)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • Experimental Validation Results for JunB Antibody (YA325)
  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20μg) , SiHa(lane 3(20μg) ,U937(lane 4(20μg)and THP-1( lane 5(20μg) using JunB Antibody (HY-P80200). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using JunB antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80200, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • JunB Antibody (YA325) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling JunB with JunB Antibody (HY-P80200) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with JunB Antibody (HY-P80200) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • JunB Antibody (YA325) Product Image WB
  • JunB Antibody (YA325) Product Image IHC-P
  • JunB Antibody (YA325) Product Image IHC-P
  • JunB Antibody (YA325) Product Image IHC-P
  • JunB Antibody (YA325) Product Image IHC-P
  • JunB Antibody (YA325) Product Image IHC-P
  • JunB Antibody (YA325) Product Image IHC-P
  • JunB Antibody (YA325) Product Image ICC
  • JunB Antibody (YA325) Product Image ICC