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Cat. No.: HY-76222

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Isatoic anhydride

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Isatoic anhydride is a single-adduct forming reagent and a monofunctional SHAPE reagent. Isatoic anhydride reacts with the 2'-hydroxyl group of RNA nucleotides via a single reactive group to form a single adduct, without forming cross-links between nucleotides. In SHAPE-JuMP experiments, Isatoic anhydride serves as a control reagent for single-adduct formation, which is used to distinguish true cross-links from single-adduct formation.

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Pureté: 99.26%
CAS No.: 118-48-9
Formule: C₈H₅NO₃
Masse moléculaire:163.13

Stockage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Isatoic anhydride Chemical Structure

CAS No.: 118-48-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Description

In Vitro

Isatoic anhydride (incubated for 15-30 min) forms monoadducts exclusively with the catalytic domain RNA of RNase P and VS ribozyme RNA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 118-48-9
Appearance Solid
Masse moléculaire 163.13
Formule C₈H₅NO₃
Color Light brown to gray
SMILES

O=C1OC(C2=C(N1)C=CC=C2)=O
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Pureté et documentation

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Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Christy TW, et al. Direct Mapping of Higher-Order RNA Interactions by SHAPE-JuMP. Biochemistry. 2021;60(25):1971-1982. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Isatoic anhydride

MCE Kinase Hinge Binder Fragment Library

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Isatoic anhydride Related Classifications

HY-76222 Related Classifications