NIR-BG2
NIR-BG2 is a fluorescent indicator targeting senescence-associated β-galactosidase (SA-β-Gal). NIR-BG2 contains a hemicyanine fluorophore caged by SA-β-Gal; cleavage of its β-galactosyl group by SA-β-Gal generates an electrophilic quinone methide, which can form covalent bonds with adjacent proteins to achieve long-term signal retention. NIR-BG2 activates a near-infrared fluorescent signal upon recognition of SA-β-Gal. NIR-BG2 can be used for non-invasive in vivo imaging of cellular senescence in mouse xenograft models. The excitation/emission wavelengths are 675/708 nm.
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- CAS No.: 3036456-18-2
- Formule: C42H46F2NO8+
- Masse moléculaire:730.81
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Stockage:
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Activité biologique
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Chemical Information
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- Pureté et documentation
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Help & FAQs
Activité biologique
Description
In Vitro
NIR-BG2 (5 μM; 10 min-4 hours) exhibits significantly higher fluorescence activation levels in camptothecin (HY-16560)-induced senescent HeLa cells and CT26.CL25 cells overexpressing β-galactosidase than in their respective control cells[2].
NIR-BG2 (5 μM; 2 h) exhibits prolonged retention and colocalization with β-galactosidase in camptothecin (HY-16560)-induced senescent HeLa cells, doxorubicin (HY-15142A) -induced senescent MDA-MB-231 cells, camptothecin-induced senescent MCF7 cells, and β-galactosidase-overexpressing CT26.CL25 cells, with excellent photostability[2].
NIR-BG2 can be activated by purified E. coli β-galactosidase in PBS buffer, resulting in a 16-fold fluorescence enhancement at 709 nm, as well as a colocalized fluorescence signal that shows a linear relationship with enzyme concentration, with Km = 9.3 μM and kcat = 14.6 s-1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:camptothecin-induced senescent HeLa cells, doxorubicin-induced senescent MDA-MB-231 cells, camptothecin-induced senescent MCF7 cells, β-galactosidase-overexpressing CT26.CL25 cells, IMR-90 cells
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Concentration:5 μM
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Incubation Time:2 hours; followed by probe washout for 0, 1, 4, 24 hours
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Result:Showed retained fluorescence signals in senescent HeLa, MDA-MB-231, and MCF7 cells after probe washout.
Showed fluorescence highly colocalized with β-galactosidase immunostaining in senescent HeLa cells.
Accumulated for more than 24 hours in CT26.CL25 cells, while control probe NIR-BG1 was cleared within 24 hours.
Showed ~15% decrease in fluorescence intensity after 30 minutes of continuous excitation in CT26.CL25 cells, indicating good photostability.
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Cell Line:HeLa, CT26.WT, CT26.CL25, MCF7, IMR-90 cells
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Concentration:0.5, 1, 2, 5, 10 μM
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Incubation Time:4 hours
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Result:Did not affect cell viability at concentrations up to 10 μM.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3036456-18-2
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Masse moléculaire 730.81
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Formule C42H46F2NO8+
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SMILES
CCC[N+]1=C(/C=C/C2=C3C(CCC2)=CC4=CC(C(F)F)=C(OCC5=CC=C(C=C5)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)C=C4O3)C(C)(C)C7=CC=CC=C17
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Zhang Y, et al. Recent advances in self-immobilizing fluorescent probes for in vivo imaging. Smart molecules : open access. 2024 Sep;2(3):e20240031. [Content Brief]
[2]. Liu J, et al. Noninvasive NIR Imaging of Senescence viaIn Situ Labeling. J Med Chem. 2021 Dec 23;64(24):17969-17978. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NIR-BG2 Related Classifications
HY-165424 Related Classifications
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