NIR-BG2
NIR-BG2 is a fluorescent indicator targeting senescence-associated β-galactosidase (SA-β-Gal). NIR-BG2 contains a hemicyanine fluorophore caged by SA-β-Gal; cleavage of its β-galactosyl group by SA-β-Gal generates an electrophilic quinone methide, which can form covalent bonds with adjacent proteins to achieve long-term signal retention. NIR-BG2 activates a near-infrared fluorescent signal upon recognition of SA-β-Gal. NIR-BG2 can be used for non-invasive in vivo imaging of cellular senescence in mouse xenograft models. The excitation/emission wavelengths are 675/708 nm.
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- CAS No.: 3036456-18-2
- Formule: C42H46F2NO8+
- Masse moléculaire:730.81
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
NIR-BG2 can be activated by purified E. coli β-galactosidase in PBS buffer, resulting in a 16-fold fluorescence enhancement at 709 nm, as well as a colocalized fluorescence signal that shows a linear relationship with enzyme concentration, with Km = 9.3 μM and kcat = 14.6 s-1[2].
NIR-BG2 (5 μM; 10 min-4 hours) exhibits significantly higher fluorescence activation levels in camptothecin (HY-16560)-induced senescent HeLa cells and CT26.CL25 cells overexpressing β-galactosidase than in their respective control cells[2].
NIR-BG2 (5 μM; 2 h) exhibits prolonged retention and colocalization with β-galactosidase in camptothecin (HY-16560)-induced senescent HeLa cells, doxorubicin (HY-15142A) -induced senescent MDA-MB-231 cells, camptothecin-induced senescent MCF7 cells, and β-galactosidase-overexpressing CT26.CL25 cells, with excellent photostability[2].
NIR-BG2 (0.5-10 μM; 4 h) shows no cytotoxicity against HeLa, CT26.WT, CT26.CL25, MCF7 and IMR-90 cells even at the highest concentration of 10 μM after 4 hours of incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:camptothecin-induced senescent HeLa cells, doxorubicin-induced senescent MDA-MB-231 cells, camptothecin-induced senescent MCF7 cells, β-galactosidase-overexpressing CT26.CL25 cells, IMR-90 cells
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Concentration:5 μM
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Incubation Time:2 hours; followed by probe washout for 0, 1, 4, 24 hours
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Result:Showed retained fluorescence signals in senescent HeLa, MDA-MB-231, and MCF7 cells after probe washout.
Showed fluorescence highly colocalized with β-galactosidase immunostaining in senescent HeLa cells.
Accumulated for more than 24 hours in CT26.CL25 cells, while control probe NIR-BG1 was cleared within 24 hours.
Showed ~15% decrease in fluorescence intensity after 30 minutes of continuous excitation in CT26.CL25 cells, indicating good photostability.
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Cell Line:HeLa, CT26.WT, CT26.CL25, MCF7, IMR-90 cells
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Concentration:0.5, 1, 2, 5, 10 μM
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Incubation Time:4 hours
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Result:Did not affect cell viability at concentrations up to 10 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3036456-18-2
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Masse moléculaire 730.81
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Formule C42H46F2NO8+
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SMILES
CCC[N+]1=C(/C=C/C2=C3C(CCC2)=CC4=CC(C(F)F)=C(OCC5=CC=C(C=C5)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)C=C4O3)C(C)(C)C7=CC=CC=C17
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Zhang Y, et al. Recent advances in self-immobilizing fluorescent probes for in vivo imaging. Smart molecules : open access. 2024 Sep;2(3):e20240031. [Content Brief]
[2]. Liu J, et al. Noninvasive NIR Imaging of Senescence viaIn Situ Labeling. J Med Chem. 2021 Dec 23;64(24):17969-17978. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NIR-BG2 Related Classifications
HY-165424 Related Classifications
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