Rapid characterization of TCM Qianjinteng by UPLC-QTOF-MS and its application in the evaluation of three species of Stephania

  • J Pharm Biomed Anal. 2018 Jul 15;156:284-296. doi: 10.1016/j.jpba.2018.04.044.
Jiao Xiao  1 ,  Nannan Song  2 ,  Tong Lu  2 ,  Yingni Pan  1 ,  Junyu Song  1 ,  Gang Chen  1 ,  Lu Sun  3 ,  Ning Li  4
Affiliations
  • 1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education, Yanbian, China.
  • 2. School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China.
  • 3. School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China. Electronic address: [email protected].
  • 4. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education, Yanbian, China. Electronic address: [email protected].
PMID: 29730338 DOI: 10.1016/j.jpba.2018.04.044
Abstract

A method for rapid identification of bioactive components in herbals from Stephania species was developed by utilizing UPLC-QTOF-MS/MS in this study. Sixty-three Alkaloids including the types of proaporphine, aporphine, protoberberine, morphine, hasubanan, benzylisoquinoline and bisbenzylisoquinoline were characterized. Among them, 29 components were selected for semi-quantitative analyses to characterize the difference of chemical compositions among three Stephania species. The results revealed that bisbenzylisoquinoline-type of Alkaloids are the representative components of SB (Subgen. Botryodiscia). The content of aporphine-type of Alkaloids was much higher in ST (Subgen. Tuberiphania) than that in SS (Subgen. Stephania), while the content of protoberberine-type of Alkaloids was higher than that in ST. The present study offered an efficient approach for quality control of Qianjinteng based on the rapid identification and semi-quantification of Alkaloids in Stephania species by using UPLC-QTOF-MS.

Keywords
Effective alkaloids; LC-QTOF-MS; OPLS-DA; Quality control; Stephania species; TCM Qianjinteng.
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