(Rac)-tris-NTA
(Rac)-tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins.
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- CAS No.: 1146592-99-5
- Formule: C43H68N8O22
- Masse moléculaire:1049.04
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
(Rac)-tris-NTA, which in presence of Ni2+ ions can interact with a His10-tag fused to the intracellular C terminus of the receptor. Whereas the (Rac)-tris-NTA group shows an affinity Kd to bind the His10-tag of ~10-30 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1146592-99-5
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Masse moléculaire 1049.04
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Formule C43H68N8O22
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SMILES
O=C(N1CCCN(CCN(CCCN(CC1)C(CCCCCN)=O)C(CCC(N(CC(O)=O)CC(O)=O)C(O)=O)=O)C(CCC(N(CC(O)=O)CC(O)=O)C(O)=O)=O)CCC(N(CC(O)=O)CC(O)=O)C(O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Moritz Pfreundschuh, et al. Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nat Commun. 2015 Nov 12;6:8857. [Content Brief]
[2]. Luyao Liu, et al. Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips. Anal Chim Acta. 2021 Jul 25;1170:338625. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)