tris-NTA
Based on 1 Customer Validation
tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 862778-60-7
- Formula: C43H68N8O22
- Molecular Weight:1049.04
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 862778-60-7
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Appearance Solid
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Molecular Weight 1049.04
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Formula C43H68N8O22
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Color White to light yellow
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SMILES
O=C(N1CCN(CCCN(CCN(CCC1)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)C(CCCCCN)=O)C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : ≥ 100 mg/mL (95.33 mM)
DMSO : 50 mg/mL (47.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (95.33 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Moritz Pfreundschuh, et al. Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nat Commun. 2015 Nov 12;6:8857. [Content Brief]
[2]. Luyao Liu, et al. Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips. Anal Chim Acta. 2021 Jul 25;1170:338625. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.9533 mL | 4.7663 mL | 9.5325 mL | 23.8313 mL |
| 5 mM | 0.1907 mL | 0.9533 mL | 1.9065 mL | 4.7663 mL | |
| 10 mM | 0.0953 mL | 0.4766 mL | 0.9533 mL | 2.3831 mL | |
| 15 mM | 0.0636 mL | 0.3178 mL | 0.6355 mL | 1.5888 mL | |
| 20 mM | 0.0477 mL | 0.2383 mL | 0.4766 mL | 1.1916 mL | |
| 25 mM | 0.0381 mL | 0.1907 mL | 0.3813 mL | 0.9533 mL | |
| 30 mM | 0.0318 mL | 0.1589 mL | 0.3178 mL | 0.7944 mL | |
| 40 mM | 0.0238 mL | 0.1192 mL | 0.2383 mL | 0.5958 mL | |
| H2O | 50 mM | 0.0191 mL | 0.0953 mL | 0.1907 mL | 0.4766 mL |
| 60 mM | 0.0159 mL | 0.0794 mL | 0.1589 mL | 0.3972 mL | |
| 80 mM | 0.0119 mL | 0.0596 mL | 0.1192 mL | 0.2979 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
tris-NTA Related Classifications
HY-D1288A Related Classifications
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