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Art. -Nr.: HY-D1721

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Fluorescein-12-dATP

Fluorescein-12-dATP is a fluorescein-labeled deoxyadenosine triphosphate and signal amplifier. Fluorescein-12-dATP can be incorporated into the 3'-end of exposed thrombin-binding aptamers via terminal deoxynucleotidyl transferase to form multi-labeled nucleic acid chains, which are used for signal amplification in thrombin detection. Fluorescein-12-dATP acts as a fluorescent detection probe in chip-based aptamer sandwich biosensors for the detection of thrombin in serum. Fluorescein-12-dATP can be used in studies related to thrombin detection.

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Formel: C₄₁H₄₁N₆O₁₉P₃
Molecular Weight:1014.71

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

Fluorescein-12-dATP Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Fluorescein-12-dATP (1-100 μM; 1 h at 37 °C), when used with terminal deoxynucleotidyl transferase to amplify signals on an aptamer-functionalized silicon chip, enables highly sensitive and selective detection of thrombin, with a linear response from 100 fM to 0.1 μM and a limit of detection of 2.0 fM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 1014.71
Formel C₄₁H₄₁N₆O₁₉P₃
SMILES

O=C(NCC#CC1=CN(C2=C1C(N)=NC=N2)[C@@]3(O[C@@H]([C@H](C3)O)COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[H])CCCCCNC(C4=CC=C(C(C(O)=O)=C4)C5=C(C(OC6=C5C=CC(O)=C6)=C7)C=CC7=O)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wen D, et al. Highly sensitive fluorometric determination of thrombin by on-chip signal amplification initiated by terminal deoxynucleotidyl transferase. Mikrochim Acta. 2018;185(8):380. Published 2018 Jul 19. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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