Art. -Nr.: HY-134570

ZINC40099027

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

ZINC40099027 is a selective FAK activator. ZINC40099027 promotes FAK phosphorylation, without activating its paralogs Pyk2 and Src. ZINC40099027 promotes the wound closure of human intestinal epithelial monolayers and the healing of mouse ulcers by activating FAK. ZINC40099027 can be used for diseases related to gastrointestinal mucosal injury research.

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Reinheit: 99.64%
CAS. Nr.: 1211825-25-0
Formel: C₂₃H₂₆F₃N₃O₃
Molecular Weight:449.47

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

ZINC40099027 Chemical Structure

CAS. Nr.: 1211825-25-0

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

ZINC40099027 (10-1000 nM, 1 h) promotes FAK phosphorylation in Caco-2 intestinal epithelial cells^[1].
ZINC40099027 (10 nM; 24 hours) accelerates the wound closure of Caco-2 epithelial monolayers^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Caco-2 intestinal epithelial cells
Concentration:

10 nM, 100 nM, 1000 nM
Incubation Time:

1 h
Result:

Specifically increased FAK-Tyr 397 phosphorylation within intact cells while activating neither Pyk2, a close paralogue of FAK, nor Src, another key nonreceptor kinase within the focal adhesion complex.

In Vivo

ZINC40099027 (900 µg/kg; intraperitoneal injection; once every 6 hours; treatment for 3 days) promotes intestinal mucosal healing, reduces the ulcer area, increases the immunoreactivity of phosphorylated FAK in the epithelium at the edge of the ulcer, and does not affect the percentage of Ki-67 positive cells in the Acetic acid (HY-Y0319)-induced ischemic ulcer model in mice^[1].
ZINC40099027 (900 µg/kg; intraperitoneal injection; once every 6 hours; treatment for 3 days) promotes ulcer healing, reduces the total ulcer area, and has no significant effect on the morphology of the kidneys and liver in the Indomethacin (HY-14397)-induced small intestine injury model in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J mice (both male and female, 8-10 weeks old), the ischemic ulcer model was induced by placing a 75% Acetic acid-saturated circular filter disk on the antimesenteric serosa of the jejunum for 15 seconds^[1]
Dosage:

900 µg/kg
Administration:

Intraperitoneal injection, once every 6 hours, for 3 days
Result:

Had smaller ulcers than those receiving the DMSO control.
The percentage of wound closure was markedly higher.
Showed higher immunoreactivity for phosphorylated FAK in the epithelium at the edge.

Animal Model:

C57BL/6J mice (both male and female, 8-10 weeks old), the Indomethacin-induced ulcer model was induced by subcutaneous injection of 15 mg/kg Indomethacin dissolved in 100% ethanol^[1]
Dosage:

900 µg/kg
Administration:

Intraperitoneal injection, once every 6 hours, for 3 days
Result:

Reduced the total ulcer area compared to the DMSO control.

Chemical Information

CAS. Nr. 1211825-25-0
Appearance Solid
Molecular Weight 449.47
Formel C₂₃H₂₆F₃N₃O₃
Color White to off-white
SMILES

O=C(N1C(C2=CC=C(OC)C=C2)CCC1)NC3=CC(C(F)(F)F)=CC=C3N4CCOCC4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

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Journal Impact Factor

Cell Death Discov

Non-enzymatic function of QSOX2 directly regulates the JUNB-ITGB4 axis and enhanced resistance to osimertinib in EGFR-mutation lung adenocarcinoma. [Abstract]

2026 Apr 1;12(1):215. PMID: 41922310
Free Radic Biol Med

Targeting DUSP26 to drive cardiac mitochondrial dynamics via FAK-ERK signaling in diabetic cardiomyopathy. [Abstract]

2024 Nov 5:225:856-870. PMID: 39510451

ZINC40099027 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 5:225:856-870. [Abstract]

ZINC40099027 (10 nM; 2 h) significantly reversed the suppression of FAK and ERK phosphorylation induced by DUSP26 overexpression in H9c2 cells exposed to GP. β-actin.

ZINC40099027 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 5:225:856-870. [Abstract]

ZINC40099027 (10 nM; 2 h) treatment led to significant changes in mitochondrial ROS and membrane potential in H9c2 cells, as evidenced by MitoSOX and JC-1 staining.

ZINC40099027 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 5:225:856-870. [Abstract]

ZINC40099027 (10 nM; 2 h), a potent FAK activator, altered basal respiration, ATP production, and maximal respiration in DUSP26-overexpressing H9c2 cells.

ZINC40099027 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 5:225:856-870. [Abstract]

ZINC40099027 (10 nM; 2 h) reversed the changes of mitochondrial dynamic proteins (Drp1, Mfn1, Mfn2, and OPA1) in H9c2 cells induced by DUSP26 overexpression.

ZINC40099027 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 5:225:856-870. [Abstract]

FAK activator ZINC40099027 (10 nM; 2 h) caused a significant increase in lipid droplet accumulation in DUSP26-overexpressing H9c2 cells under glucolipotoxic conditions.
J Funct Foods

2024 May, 116, 106224.
SSRN

2025 Dec 12.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (222.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2248 mL

11.1242 mL

22.2484 mL

5 mM

0.4450 mL

2.2248 mL

4.4497 mL

10 mM

0.2225 mL

1.1124 mL

2.2248 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (5.56 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (5.56 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang Q, et al. Small molecule FAK activator promotes human intestinal epithelial monolayer wound closure and mouse ulcer healing. Sci Rep. 2019 Oct 11;9(1):14669. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2248 mL	11.1242 mL	22.2484 mL	55.6211 mL
	5 mM	0.4450 mL	2.2248 mL	4.4497 mL	11.1242 mL
	10 mM	0.2225 mL	1.1124 mL	2.2248 mL	5.5621 mL
	15 mM	0.1483 mL	0.7416 mL	1.4832 mL	3.7081 mL
	20 mM	0.1112 mL	0.5562 mL	1.1124 mL	2.7811 mL
	25 mM	0.0890 mL	0.4450 mL	0.8899 mL	2.2248 mL
	30 mM	0.0742 mL	0.3708 mL	0.7416 mL	1.8540 mL
	40 mM	0.0556 mL	0.2781 mL	0.5562 mL	1.3905 mL
	50 mM	0.0445 mL	0.2225 mL	0.4450 mL	1.1124 mL
	60 mM	0.0371 mL	0.1854 mL	0.3708 mL	0.9270 mL
	80 mM	0.0278 mL	0.1391 mL	0.2781 mL	0.6953 mL
	100 mM	0.0222 mL	0.1112 mL	0.2225 mL	0.5562 mL

Keywords

ZINC40099027

4 Cited Publications

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ZINC40099027

Immunology/Inflammation Compound Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Highly Selective Activators Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ZINC40099027

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ZINC40099027

Immunology/Inflammation Compound Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Highly Selective Activators Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ZINC40099027

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ZINC40099027 Related Classifications

HY-134570 Related Classifications