Zinpyr-1
Based on 4 publication(s) in Google Scholar
Zinpyr-1 is a zinc-responsive fluorescent indicator and a membrane-permeable metal-binding probe. Zinpyr-1 forms a complex with Mn2+ ions and generates a fluorescence turn-on signal. Zinpyr-1 binds to free zinc ions in serum, enabling quantitative detection of free zinc concentration. Zinpyr-1 produces fluorescence signals reflecting the relative zinc concentration in plant root cells, localizes to specific layers of plant root cells, and can be used to support analyses related to plant zinc transporter mutations and homeostasis.
For research use only. We do not sell to patients.
- Purity: 95.85%
- CAS No.: 288574-78-7
- Formula: C46H36Cl2N6O5
- Molecular Weight:823.72
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Zinpyr-1
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Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Zinpyr-1 (10 μM; pH 6-8) forms a 1:2 complex with Mn2+. Compared to metal-free Zinpyr-1, this complex exhibits a 25-fold enhanced fluorescence dynamic range for Zn2+ detection (106 at pH 7.0). It also maintains a high dynamic range at pH 6.0 and 8.0, and shows better selectivity for Zn2+ over the abundant monovalent and divalent cations in organisms[1].
Zinpyr-1 (10 μM) forms a Mn2+ complex that can penetrate cell membranes, exhibits no toxicity to HeLa cells, has extremely low background fluorescence, and shows strong activation-type fluorescence responsive to zinc ions, making it suitable for live-cell imaging[1].
Zinpyr-1 (0.1 mM) forms a Mn2+ complex that functions as a zinc-responsive MRI contrast agent[1].
Zinpyr-1 stably induces the minimum fluorescence (Fmin) and maximum fluorescence (Fmax) required for free zinc quantification in serum, with the maximum excitation and emission wavelengths being 507 nm and 526 nm, respectively, enabling reliable quantification of free zinc in serum samples[2].
Zinpyr-1 (5-20 μM; 15 min-3 h) serves as an effective sensor for relative zinc levels in root cells of Arabidopsis thaliana seedlings, and its fluorescence intensity reflects intracellular zinc concentrations[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 288574-78-7
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Appearance Solid
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Molecular Weight 823.72
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Formula C46H36Cl2N6O5
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Color Orange to red
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(Cl)C(O)=C4CN(CC5=NC=CC=C5)CC6=NC=CC=C6)C(CN(CC7=NC=CC=C7)CC8=NC=CC=C8)=C(O)C(Cl)=C3)C9=C1C=CC=C9
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (4)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Colistin-induced acute kidney injury via zinc Dyshomeostasis-triggered GSDMD-executed Pyroptosis. [Abstract]2026 Jan 1;168(Pt 1):115768. PMID: 41176907 -
Sci Rep
Disruption of zinc homeostasis reduces histone acetylation levels in normal and tumor cells. [Abstract]2026 Jan 10;16(1):4983. PMID: 41519875
Zinpyr-1 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jan 10;16(1):4983. [Abstract]
The distribution of free zinc ions in HL-1 with or without pyrithione after 4 h of treatment with zinc. Pyrithione, zinc ionophores, 20 µM. HL-1 cells labeled with 5 µM Zinpyr-1 for 0.5 h at 37℃.
Zinpyr-1 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jan 10;16(1):4983. [Abstract]
The distribution of free zinc ions in AC16 cells after treatment with ZnSO4. AC16 cells labeled with 5 µM Zinpyr-1 for 0.5 h at 37℃. Scale bar, 20 µm.
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Zinpyr-1 purchased from MedChemExpress. Usage Cited in: Explor BioMat-X. 2025 Jul 7;2:101341.
Morselized human allograft was treated with 0.5% (w/v) ZnCl2 dissolved in TBS buffered to pH 7.4 for 60 min and then washed extensively with TBS before detecting bound Zn with the fluorescent stain, Zinpyr-1. The stained allograft morsels were polished to reveal the interior of the bone morsels before epi-fluorescent detection of the Zinpyr-1. Shown are two, approximately 1 mm diameter allograft morsels; scale bars are 200 µm.
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Solvent & Solubility
DMSO : 20 mg/mL (24.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. You Y, et al. Manganese displacement from Zinpyr-1 allows zinc detection by fluorescence microscopy and magnetic resonance imaging. Chem Commun (Camb). 2010;46(23):4139-4141. [Content Brief]
[2]. Alker W, et al. A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum. Int J Mol Sci. 2019;20(16):4006. Published 2019 Aug 16. [Content Brief]
[3]. Sinclair SA, et al. The use of the zinc-fluorophore, Zinpyr-1, in the study of zinc homeostasis in Arabidopsis roots. New Phytol. 2007;174(1):39-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2140 mL | 6.0700 mL | 12.1400 mL | 30.3501 mL |
| 5 mM | 0.2428 mL | 1.2140 mL | 2.4280 mL | 6.0700 mL | |
| 10 mM | 0.1214 mL | 0.6070 mL | 1.2140 mL | 3.0350 mL | |
| 15 mM | 0.0809 mL | 0.4047 mL | 0.8093 mL | 2.0233 mL | |
| 20 mM | 0.0607 mL | 0.3035 mL | 0.6070 mL | 1.5175 mL |
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