Coumarin 6
Based on 24 publication(s) in Google Scholar
Coumarin 6, a fluorescent dye, is used as a fluorescent probe in a microparticle drug delivery system to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems (λexc=450 nm, λem=505 nm).
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 99.59%
- CAS No.: 38215-36-0
- Formule: C20H18N2O2S
- Masse moléculaire:350.44
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Coumarin 6
More- Bioact Mater. 2023 Apr 19:27:337-347. [Abstract]
- ACS Nano. 2025 Mar 18;19(10):10433-10451. [Abstract]
- J Nanobiotechnology. 2025 Mar 22;23(1):237. [Abstract]
- Acta Pharm Sin B. 2026 Apr 24.
- Chem Eng J. 2025 Aug 25.
- Chem Eng J. 2025 Sep 15.
- Chem Eng J. 2024 Feb 1, 481, 148614.
- J Control Release. 2025 May 25:384:113889. [Abstract]
- Phytomedicine. 2025 May 30:144:156917. [Abstract]
- Phytomedicine. 2025 Apr:139:156514. [Abstract]
- Mater Today Bio. 2025 Dec 5:35:102635. [Abstract]
- Mater Today Bio. 2025 May 4:32:101825. [Abstract]
- Acta Biomater. 2025 Aug:202:503-516. [Abstract]
- J Colloid Interface Sci. 2026 Feb 15;704(Pt 2):139399. [Abstract]
- Int J Biol Macromol. 2025 Jun 14:145221. [Abstract]
- Int J Nanomedicine. 2024 Aug 1:19:7831-7850. [Abstract]
- Int J Biol Macromol. 2024 May;266(Pt 1):130637. [Abstract]
- Nanoscale Adv. 2024 Jun 14;6(16):4082-4093. [Abstract]
- Cancer Nanotechnol. 2023 May 9.
- ACS Omega. 2026 Apr 22;11(17):25025-25033. [Abstract]
- Eur J Pharm Biopharm. 2026 Jul:224:115081. [Abstract]
- bioRxiv. 2026 Jan 30.
- SSRN. 2025 Jul 26.
- Biomed Pharmacother. 2024 Aug 5:178:116992. [Abstract]
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Flow Cytometry
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In Vivo Imaging
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Others
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16 | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against mouse B16 cell line
Antiproliferative activity against mouse B16 cell line
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[PMID: 16793260] |
| DLD-1 | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against human DLD1 cell line
Antiproliferative activity against human DLD1 cell line
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[PMID: 16793260] |
| HeLa | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against human HeLa cell line
Antiproliferative activity against human HeLa cell line
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[PMID: 16793260] |
| HUVEC | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against HUVEC cell line
Antiproliferative activity against HUVEC cell line
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[PMID: 16793260] |
| NIH3T3 | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against mouse NIH3T3 cell line
Antiproliferative activity against mouse NIH3T3 cell line
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[PMID: 16793260] |
| SiHa | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against human SiHa cell line
Antiproliferative activity against human SiHa cell line
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[PMID: 16793260] |
| U-87MG ATCC | IC50 |
>70 μM
Compound: 11
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Antiproliferative activity against human U87 MG cell line
Antiproliferative activity against human U87 MG cell line
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[PMID: 16793260] |
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Coumarin 6 labeled HPMC nanoparticles[3]
1. Dissolve Coumarin 6 in DMF to prepare a 5 mg/mL organic phase solution.
2. Dissolve hydroxypropyl methyl cellulose (HPMC) in HBSS to prepare a 50 μg/mL aqueous solution.
3. Add the organic solution to the aqueous solution using a MasterFlex L/S pump (volume ratio of organic solution to aqueous solution is 1:100) with an injection rate of 36 mL/min.
4. Prepare 70 nm nanoparticles by stirring at room temperature for 5 min at a stirring speed of 9 mL/min.
5. Add 2 μg/mL nanoparticles to the MDCKII cell culture dish and incubate in the dark for 5 min, 15 min, 30 min and 60 min.
6. Add cold PBS buffer to stop the uptake of nanoparticles by MDCKII cells and clean with HBSS.
7. Use a fluorescence microscope to observe the uptake of nanoparticles by MDCKII cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
505
450
Chemical Information
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CAS No. 38215-36-0
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Appearance Solid
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Masse moléculaire 350.44
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Formule C20H18N2O2S
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Color Yellow to orange
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SMILES
O=C1C(C2=NC3=CC=CC=C3S2)=CC4=CC=C(N(CC)CC)C=C4O1
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (24)
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Journal Impact Factor
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Most Recent
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Bioact Mater
A homologous and molecular dual-targeted biomimetic nanocarrier for EGFR-related non-small cell lung cancer therapy. [Abstract]2023 Apr 19:27:337-347. PMID: 37122898
Coumarin 6 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2023 Apr 19:27:337-347. [Abstract]
Coumarin 6 (C 6). Confocal laser scanning microscopy images of HCC827 cells coincubated with dual fluorescent dye-labeled CMNP@Osi.
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ACS Nano
Vitamin A-Integrated Cinnamaldehyde Nanoemulsion: A Nanotherapeutic Approach To Counteract Liver Fibrosis via Gut-Liver Axis Modulation. [Abstract]2025 Mar 18;19(10):10433-10451. PMID: 40045827 -
J Nanobiotechnology
Recombinant high-density lipoprotein targeted delivery of celastrol to promote foam cells lipophagy against early atherosclerosis. [Abstract]2025 Mar 22;23(1):237. PMID: 40119460
Coumarin 6 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Mar 22;23(1):237. [Abstract]
Quantitative analysis of C6-rHDL uptake and Coumarin 6 fluorescence intensity in foam cells.
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J Control Release
Synergistic blockade of SHP-2 and A2AR signal pathways with targeted nanoparticles restores anti-tumor immunity of CD8+ T cells. [Abstract]2025 May 25:384:113889. PMID: 40425093
Coumarin 6 purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 May 25:384:113889. [Abstract]
The fluorescence microscope images for cellular internalization of Coumarin 6 (C6)-labeled nanoparticles. Scale bar: 50 μm.
Coumarin 6 purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 May 25:384:113889. [Abstract]
Flow cytometry was used to quantitatively analyze the cellular internalization of nanoparticles labeled with the dye (Coumarin 6).
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Phytomedicine
Ginsenoside F2-modified liposomes delivering FTY720 enhance glioblastoma targeting and antitumor activity via ferroptosis. [Abstract]2025 May 30:144:156917. PMID: 40480023 -
Phytomedicine
Celastrol-loaded ginsenoside Rg3 liposomes enhance anti-programmed death ligand 1 immunotherapy by inducing immunogenic cell death in triple-negative breast cancer. [Abstract]2025 Apr:139:156514. PMID: 39986227 -
Mater Today Bio
PH20-modified exosomes loaded curcumin inhibit desmoplastic breast cancer by breaking extracellular matrix barrier and normalizing cancer-associated fibroblasts. [Abstract]2025 Dec 5:35:102635. PMID: 41458179
Coumarin 6 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 5:35:102635. [Abstract]
Fluorescent distribution of C6 away from blood vessels in tumor resection specimens after injection of free C6 (Coumarin 6) and C6-labeled nanoparticles.
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Mater Today Bio
TMTP1-modified polymeric micelles for the inhibition of ovarian cancer metastasis and recurrence through enhanced photothermal-immunotherapy. [Abstract]2025 May 4:32:101825. PMID: 40487160 -
Acta Biomater
A ROS/ultrasound dual-responsive nanocarrier enhances drug penetration for ameliorating metabolic dysfunction-associated steatohepatitis. [Abstract]2025 Aug:202:503-516. PMID: 40617493 -
J Colloid Interface Sci
Design and mechanistic insights of diacylglycerol-based microemulsions for enhanced transdermal delivery: experimental optimization and molecular dynamics simulation. [Abstract]2026 Feb 15;704(Pt 2):139399. PMID: 41205530 -
Int J Biol Macromol
pPB peptide-modified nanostructured lipid carriers loaded with astragaloside IV as a potential strategy for enhanced antifibrosis therapy. [Abstract]2025 Jun 14:145221. PMID: 40523486 -
Int J Nanomedicine
Bufalin-Loaded Multifunctional Photothermal Nanoparticles Inhibit the Anaerobic Glycolysis by Targeting SRC-3/HIF-1α Pathway for Improved Mild Photothermal Therapy in CRC. [Abstract]2024 Aug 1:19:7831-7850. PMID: 39105099 -
Int J Biol Macromol
Engineering of VCAM-1-targeted nanostructured lipid carriers for delivery of melatonin against acute lung injury through SIRT1/NLRP3 mediated pyroptosis signaling pathway. [Abstract]2024 May;266(Pt 1):130637. PMID: 38490396 -
Nanoscale Adv
Nanoparticle co-delivery of carboplatin and PF543 restores platinum sensitivity in ovarian cancer models through inhibiting platinum-induced pro-survival pathway activation. [Abstract]2024 Jun 14;6(16):4082-4093. PMID: 39114142 -
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ACS Omega
HDACi-Loaded Nanoliposomes Enhance the Diversity of Antibody CDRs in Rabbits and the Application in Antibody Preparation. [Abstract]2026 Apr 22;11(17):25025-25033. PMID: 42110727 -
Eur J Pharm Biopharm
Spatiotemporally controlled systemic delivery reshapes the in vivo fate of a cationic antimicrobial peptide for lung-selective exposure and an improved therapeutic index. [Abstract]2026 Jul:224:115081. PMID: 42044857 -
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Biomed Pharmacother
Targeted-lung delivery of bardoxolone methyl using PECAM-1 antibody-conjugated nanostructure lipid carriers for the treatment of lung inflammation. [Abstract]2024 Aug 5:178:116992. PMID: 39106709
Solvant et solubilité
THF : 12.5 mg/mL (35.67 mM; Need ultrasonic)
DMSO : 5 mg/mL (14.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[2]. Rivolta I, et al. Cellular uptake of coumarin-6 as a model drug loaded in solid lipid nanoparticles. J Physiol Pharmacol. 2011 Feb;62(1):45-53. [Content Brief]
[3]. Miao X, et al. Transport Mechanism of Coumarin 6 Nanocrystals with Two Particle Sizes in MDCKII Monolayer and Larval Zebrafish. ACS Appl Mater Interfaces. 2016 May 25;8(20):12620-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / THF | 1 mM | 2.8536 mL | 14.2678 mL | 28.5356 mL | 71.3389 mL |
| 5 mM | 0.5707 mL | 2.8536 mL | 5.7071 mL | 14.2678 mL | |
| 10 mM | 0.2854 mL | 1.4268 mL | 2.8536 mL | 7.1339 mL | |
| THF | 15 mM | 0.1902 mL | 0.9512 mL | 1.9024 mL | 4.7559 mL |
| 20 mM | 0.1427 mL | 0.7134 mL | 1.4268 mL | 3.5669 mL | |
| 25 mM | 0.1141 mL | 0.5707 mL | 1.1414 mL | 2.8536 mL | |
| 30 mM | 0.0951 mL | 0.4756 mL | 0.9512 mL | 2.3780 mL |
Coumarin 6 Related Classifications
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