Cyanine 3 Tyramide methyl indole
Based on 1 Customer Validation
Cyanine 3 Tyramide methyl indole is a derivative of Cyanine 3 Tyramide (HY-136248). Cyanine 3 Tyramide is an orange fluorescent dye, and is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
For research use only. We do not sell to patients.
- Purity : 98.67%
- Formula: C38H45N3O8S2
- Molecular Weight:735.91
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Biological Activity
Description
Chemical Information
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Appearance Solid
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Molecular Weight 735.91
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Formula C38H45N3O8S2
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Color Brown to dark brown
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SMILES
O=S(C1=CC2=C([N+](CCCCCC(NCCC3=CC=C(O)C=C3)=O)=C(/C=C/C=C4N(C)C5=C(C=C(S(=O)(O)=O)C=C5)C/4(C)C)C2(C)C)C=C1)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Protocols
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In situ hybridization
The labeled nucleic acid probe is hybridized with the DNA or RNA on tissues and cells by using the complementary base sequence between the single strands of nucleic acid molecules. The detected DNA or RNA molecules in situ in cells can be displayed by autoradiography, fluorescence detection or enzyme color development.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Chao J, et, al. Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD). Cytometry. 1996 Jan 1;23(1):48-53. [Content Brief]
[2]. Schmidt BF, et, al. Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J Histochem Cytochem. 1997 Mar;45(3):365-73. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)