1. Cell Cycle/DNA Damage Others
  2. DNA Stain Fluorescent Dye
  3. Cyanine 3 Tyramide

Cyanine 3 Tyramide  (Synonyms: Tyramide-Cy3)

Cat. No.: HY-136248 Purity: 96.15%
COA Handling Instructions

Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.

For research use only. We do not sell to patients.

Cyanine 3 Tyramide Chemical Structure

Cyanine 3 Tyramide Chemical Structure

CAS No. : 174961-75-2

Size Price Stock Quantity
1 mg USD 220 In-stock
5 mg USD 720 In-stock
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50 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

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Description

Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids[1][2].

Molecular Weight

749.94

Formula

C39H47N3O8S2

CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

570

Excitation (Ex)

550

SMILES

O=S(C1=CC2=C([N+](CCCCCC(NCCC3=CC=C(O)C=C3)=O)=C(/C=C/C=C4N(CC)C5=C(C=C(S(=O)(O)=O)C=C5)C/4(C)C)C2(C)C)C=C1)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (27.78 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3334 mL 6.6672 mL 13.3344 mL
5 mM 0.2667 mL 1.3334 mL 2.6669 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3334 mL 6.6672 mL 13.3344 mL 33.3360 mL
5 mM 0.2667 mL 1.3334 mL 2.6669 mL 6.6672 mL
10 mM 0.1333 mL 0.6667 mL 1.3334 mL 3.3336 mL
15 mM 0.0889 mL 0.4445 mL 0.8890 mL 2.2224 mL
20 mM 0.0667 mL 0.3334 mL 0.6667 mL 1.6668 mL
25 mM 0.0533 mL 0.2667 mL 0.5334 mL 1.3334 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cyanine 3 Tyramide
Cat. No.:
HY-136248
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