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Art. -Nr.: HY-126222

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MitoTam bromide, hydrobromide

MitoTam bromide, hydrobromide, a Tamoxifen derivative, is an electron transport chain (ETC) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology. MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells.

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CAS. Nr.: 1634624-73-9
Formel: C₅₂H₆₀Br₂NOP
Molecular Weight:905.82

Speicherung:

4°C, stored under nitrogen, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

MitoTam bromide, hydrobromide Chemical Structure

CAS. Nr.: 1634624-73-9

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

MitoTam bromide, hydrobromide, a Tamoxifen derivative^[1], is an electron transport chain (ETC) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology^[2]. MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells^[1]^[2].

In Vitro

MitoTam (0.5 μM-56 μM; 24 hours) kills breast cancer cell Lines and nonmalignant cells with an IC₅₀ range from 0.65 μM to 55.9 μM^[1].
MitoTam (2.5 μM; 2-24 hours) results in stronger activation of the apoptotic pathway in MCF7 Her2 high cells compared with mock MCF7 cells^[1].
MitoTam (0.05 μM-1 μM; 3 days) causes a concentration-dependent induction of apoptosis in breast cancer cells, while there was no effect for non-malignant breast epithelial cells^[2]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Breast Cancer Cell Lines: BT474, MCF7, MCF7 Her2^high, MCF7 Her2^low, MDA-MB-231, MDA-MB-436, MDA-MB-453, SK-BR-3, T47D; NeuTL cells; Nonmalignant Cells: A014578, H9c2 cells
Concentration:

0.5 μM-56 μM
Incubation Time:

24 hours
Result:

Killed breast cancer cells MCF7, MCF7 Her2^high, MCF7 Her2^low with IC₅₀ values of 1.25 μM, 0.65 μM and 1.45 μM respectively.

Western Blot Analysis^[1]

Cell Line:

MCF7 mock cells, MCF7 Her2^high cells
Concentration:

2.5 μM
Incubation Time:

2 hours, 4 hours, 8 hours, 16 hours, 24 hours
Result:

Revealed accelerated cleavage of procaspase-9, Parp1/2 and proapoptotic Bax, decreased the antiapoptotic Bcl-2 protein in Her2high cells.

Apoptosis Analysis^[2]

Cell Line:

MCF-7 cells, 4T1 cells and MCF-10a cells
Concentration:

0.05 μM-1 μM
Incubation Time:

3 days
Result:

Resulted in apoptosis in MCF7 and 4T1 cells.

In Vivo

MitoTam (intraperitoneal injection; 2 μg/g; once a week; 4 weeks) decreases β-gal staining of lungs from MitoTam-treated mice, accompaning by a inhibition in the expression of senescence markers p16Ink4a, p21waf1 and PAI comparing control mice sup>[2].
MitoTam (intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks) inhibits growth of syngeneic tumors by 80%^[1].
MitoTam (intraperitoneal injection; 0.25 μmol/mouse; twice a week; 2 weeks) slows down the growth of MCF7 mock tumors and stops tumor progression after two doses; suppresses Her2^high carcinomas decreased threefold from the original size with complete disappearance^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

18-month-old or 2-month-old FVB/N mice^[2]
Dosage:

2 μg/g
Administration:

Intraperitoneal injection; 2 μg/g; once a week; 4 weeks
Result:

Eliminated senescent cells also in vivo.

Animal Model:

FVB/N c-neu mouse^[1]
Dosage:

0.54 μmol/mouse
Administration:

Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks
Result:

Suppressed Her2^high breast carcinomas.

Animal Model:

Balb/c nude mice with MCF7 mock or MCF7 Her2^high cells^[1]
Dosage:

0.25 μmol/mouse/dose
Administration:

Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks
Result:

Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2^high carcinomas decreased.

Chemical Information

CAS. Nr. 1634624-73-9
Appearance Solid
Molecular Weight 905.82
Formel C₅₂H₆₀Br₂NOP
Color White to off-white
SMILES

CN(CCOC1=CC=C(/C(C2=CC=CC=C2)=C(C3=CC=CC=C3)/CCCCCCCCCC[P+](C4=CC=CC=C4)(C5=CC=CC=C5)C6=CC=CC=C6)C=C1)C.Br[H].[Br-]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, stored under nitrogen, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 240 mg/mL (264.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1040 mL

5.5199 mL

11.0397 mL

5 mM

0.2208 mL

1.1040 mL

2.2079 mL

10 mM

0.1104 mL

0.5520 mL

1.1040 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Rohlenova K, et al. Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2highBreast Cancer. Antioxid Redox Signal. 2017 Jan 10;26(2):84-103. [Content Brief]

[2]. Hubackova S, et al. Selective elimination of senescent cells by mitochondrial targeting is regulated by ANT2. Cell Death Differ. 2019 Jan;26(2):276-290. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1040 mL	5.5199 mL	11.0397 mL	27.5993 mL
	5 mM	0.2208 mL	1.1040 mL	2.2079 mL	5.5199 mL
	10 mM	0.1104 mL	0.5520 mL	1.1040 mL	2.7599 mL
	15 mM	0.0736 mL	0.3680 mL	0.7360 mL	1.8400 mL
	20 mM	0.0552 mL	0.2760 mL	0.5520 mL	1.3800 mL
	25 mM	0.0442 mL	0.2208 mL	0.4416 mL	1.1040 mL
	30 mM	0.0368 mL	0.1840 mL	0.3680 mL	0.9200 mL
	40 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6900 mL
	50 mM	0.0221 mL	0.1104 mL	0.2208 mL	0.5520 mL
	60 mM	0.0184 mL	0.0920 mL	0.1840 mL	0.4600 mL
	80 mM	0.0138 mL	0.0690 mL	0.1380 mL	0.3450 mL
	100 mM	0.0110 mL	0.0552 mL	0.1104 mL	0.2760 mL

Keywords

MitoTam bromide, hydrobromide

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

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MitoTam bromide, hydrobromide

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MitoTam bromide, hydrobromide Related Classifications

HY-126222 Related Classifications