Art. -Nr.: HY-149394

PRDX1-IN-1

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

PRDX1-IN-1 is a selective inhibtor of PRDX1 with an IC₅₀ value of 0.164 μM. PRDX1-IN-1 can be used in researches related to cancer.PRDX1-IN-1 promots intracellular ROS accumulation, and inhibits the proliferation, invasion and migration of cancer cells besides inducing apoptosis. PRDX1-IN-1 could be used in cancer research.

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Reinheit: 98.04%
CAS. Nr.: 2996096-63-8
Formel: C₄₆H₅₅N₃O₄
Molecular Weight:713.95

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

PRDX1-IN-1 Chemical Structure

CAS. Nr.: 2996096-63-8

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50:0.164μM(potent peroxiredoxin 1,PRDX1)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.92 μM Compound: 7e	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 37499289]
MDA-MB-231	IC₅₀	2.67 μM Compound: 7e	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 37499289]
NCI-H1975	IC₅₀	1.99 μM Compound: 7e	Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 37499289]
SK-HEP1	IC₅₀	2.42 μM Compound: 7e	Antiproliferative activity against human SK-HEP1 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human SK-HEP1 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 37499289]

In Vitro

PRDX1-IN-1 inhibites the proliferation activities of the human lung cancer cells A549, lung cancer cell lines (LTEP-a-2 and H1975), human breast cancer cell line (MDA-MB-231), human hepatoma cell line (SK-Hep-1) with the IC₅₀ values of 1.92 μM, 2.93 μM, 1.99 μM, 2.67 μM, 2.42μM, respectively^[1].
PRDX1-IN-1 (compound 7e)(2 μM or 4 μM, 24 h) promotes intracellular ROS accumulation, and inhibits the invasion and migration of human lung cancer cells A549^[1].
PRDX1-IN-1 (2 μM or 4 μM, 24 h) induces the apoptosis of A549 cells^[1].
PRDX1-IN-1 (2 μM or 4 μM, 6 h) suppresses the key signaling pathways (AKT and ERK) and promotes the expression of apoptosis-related proteins (cleaved caspase-3/8 and cleaved PARP) in A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Proliferation assay^[1]

Cell Line:

lung cancer cell lines (LTEP-a-2 and H1975), human breast cancer cell line (MDA-MB-231), human hepatoma cell line (SK-Hep-1)
Concentration:

0.5–10 μM
Incubation Time:

48 h
Result:

Inhibited the proliferation activities of cancer cells A549, LTEP-a-2, H1975, MDA-MB-231, SK-Hep-1.

Apoptosis assay ^[1]

Cell Line:

human lung cancer cells A549
Concentration:

2 μM or 4 μM
Incubation Time:

24 h
Result:

Increased the ratio of the total number of early (annexin-V+/PI− ) and late (annexin-V+/PI+) apoptotic cells significantly.

WB assay^[1]

Cell Line:

A549 cell
Concentration:

2 μM or 4 μM
Incubation Time:

6 h
Result:

Decreased the phosphorylation levels of PI3K, AKT, C-RAF and ERK.

Matrigel invasion assay^[1]

Cell Line:

A549 cell
Concentration:

2 μM or 4 μM
Incubation Time:

24 - 48 h
Result:

Inhibted the cell matrigel and invasion.

In Vivo

PRDX1-IN-1 (0.5 or 1 mg/kg, intraperitoneal(i.p.), 19 days, every day) inhibited tumor growth in a mouse model of lung cancer^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J male mice injected with Lewis cell (lung cancer)^[1]
Dosage:

0.5 or 1 mg/kg
Administration:

intraperitoneal injection (i.p.), every day for 19 days.
Result:

Inhibited tumor growth, with the tumor growth inhibition (TGI) values of 77.47% and 69.89% in the groups of 1 mg/kg and 0.5 mg/kg, respectively.
Induced the changes in morphological characteristics of tumor cells, including cell agglutination, contraction, and nuclear chromatin marginalization.

Chemical Information

CAS. Nr. 2996096-63-8
Appearance Solid
Molecular Weight 713.95
Formel C₄₆H₅₅N₃O₄
Color Light yellow to yellow
SMILES

O=C(OC1=C(C)C2=CC=C([C@](CC[C@]3(C)[C@@]4([H])C[C@@](C(NCC5=C(C)N=C(C)C(C)=N5)=O)(C)CC3)(C)[C@@]4(C)CC6)[C@]6(C)C2=CC1=O)/C=C/C7=CC=CC=C7
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Free Radic Biol Med

Peroxiredoxin-1 aggravates hypoxia-induced renal injury by promoting inflammation through the TLR4/MAPK/NF-κB signaling pathway. [Abstract]

2025 Aug 16:236:176-187. PMID: 40398686
Exp Cell Res

Exosomes derived from diabetic microenvironment-preconditioned mesenchymal stem cells ameliorate nonalcoholic fatty liver disease and inhibit pyroptosis of hepatocytes. [Abstract]

2024 Nov 7;443(2):114325. PMID: 39521106

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (140.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4007 mL

7.0033 mL

14.0066 mL

5 mM

0.2801 mL

1.4007 mL

2.8013 mL

10 mM

0.1401 mL

0.7003 mL

1.4007 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (3.50 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (3.50 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ying Bai, et al. Development of novel celastrol-ligustrazine hybrids as potent peroxiredoxin 1 inhibitors against lung cancer. Eur J Med Chem. 2023, 259, 115656. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4007 mL	7.0033 mL	14.0066 mL	35.0165 mL
	5 mM	0.2801 mL	1.4007 mL	2.8013 mL	7.0033 mL
	10 mM	0.1401 mL	0.7003 mL	1.4007 mL	3.5016 mL
	15 mM	0.0934 mL	0.4669 mL	0.9338 mL	2.3344 mL
	20 mM	0.0700 mL	0.3502 mL	0.7003 mL	1.7508 mL
	25 mM	0.0560 mL	0.2801 mL	0.5603 mL	1.4007 mL
	30 mM	0.0467 mL	0.2334 mL	0.4669 mL	1.1672 mL
	40 mM	0.0350 mL	0.1751 mL	0.3502 mL	0.8754 mL
	50 mM	0.0280 mL	0.1401 mL	0.2801 mL	0.7003 mL
	60 mM	0.0233 mL	0.1167 mL	0.2334 mL	0.5836 mL
	80 mM	0.0175 mL	0.0875 mL	0.1751 mL	0.4377 mL
	100 mM	0.0140 mL	0.0700 mL	0.1401 mL	0.3502 mL

Keywords

PRDX1-IN-1

2 Cited Publications

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PRDX1-IN-1

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) PRDX1-IN-1

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

PRDX1-IN-1

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) PRDX1-IN-1

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

PRDX1-IN-1 Related Classifications

HY-149394 Related Classifications