Disoxaril (Synonyms: WIN-51711)

Name: Disoxaril
Brand: MedChemExpress
SKU: HY-111233
Rating: 4.5 (0 reviews)

Cat. No.: HY-111233 Purity: 96.50%: Data Sheet
COA

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Disoxaril (WIN-51711) is an antivirus agent with activity against poliovirus types 1 and 2. Disoxaril inserts into the hydrophobic interior of virus capsid protein VP1 to stabilize virion conformation, inhibits poliovirus uncoating, and prevents viral RNA release and synthesis. Disoxaril can be used for the research of poliovirus infection.

For research use only. We do not sell to patients.

Disoxaril Chemical Structure

CAS No. : 87495-31-6

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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500 mg	Get quote

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	CC₅₀	21 μM Compound: Disoxaril	Antiviral activity against Human rhinovirus type 14 in human HeLa Ohio-1 cells Antiviral activity against Human rhinovirus type 14 in human HeLa Ohio-1 cells	[PMID: 18590964]
HeLa	CC₅₀	37 μM Compound: WIN-51711	Compound concentration required to reduce the viability of mock-infected HeLa cells by 50%, was reported. Compound concentration required to reduce the viability of mock-infected HeLa cells by 50%, was reported.	[PMID: 7877145]
HeLa	EC₅₀	1.76 μM Compound: WIN-51711	In vitro concentration required to achieve 50% protection of HeLa cells from HRV-14 induced cytopathic effect In vitro concentration required to achieve 50% protection of HeLa cells from HRV-14 induced cytopathic effect	[PMID: 7877145]
HeLa	EC₅₀	2.35 μM Compound: WIN-51711	In vitro concentration required to achieve 50% protection of HeLa cells from HRV-2 induced cytopathic effect In vitro concentration required to achieve 50% protection of HeLa cells from HRV-2 induced cytopathic effect	[PMID: 7877145]
HeLa	IC₅₀	1.5 μM Compound: Disoxaril	Cytotoxicity against human HeLa Ohio-1 cells Cytotoxicity against human HeLa Ohio-1 cells	[PMID: 18590964]
RD	CC₅₀	167.39 μM Compound: 5; WIN 51711	Cytotoxicity against human RD cells assessed as reduction in cell viability after 24 to 36 hrs by MTS-based CPE reduction assay Cytotoxicity against human RD cells assessed as reduction in cell viability after 24 to 36 hrs by MTS-based CPE reduction assay	[PMID: 26885567]
RD	EC₅₀	15.32 μM Compound: 5; WIN 51711	Antiviral activity against Enterovirus 71 infected in human RD cells after 24 to 36 hrs by MTS-based CPE reduction assay Antiviral activity against Enterovirus 71 infected in human RD cells after 24 to 36 hrs by MTS-based CPE reduction assay	[PMID: 26885567]
Vero	CC₅₀	147.77 μM Compound: 5; WIN 51711	Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability after 24 to 36 hrs by MTS-based CPE reduction assay Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability after 24 to 36 hrs by MTS-based CPE reduction assay	[PMID: 26885567]
Vero	EC₅₀	19.48 μM Compound: 5; WIN 51711	Antiviral activity against Human enterovirus 71 infected in monkey African green Vero cells by MTS-based CPE reduction assay Antiviral activity against Human enterovirus 71 infected in monkey African green Vero cells by MTS-based CPE reduction assay	[PMID: 26885567]

In Vitro

Disoxaril (35-140 μM; 48 h) inhibits FL cell growth with a CGIC₅₀ of 70 μM^[1].
Disoxaril (0.03-0.3 μg/mL; 15 min preincubation) inhibits replication of poliovirus type 1 and type 2 in HeLa cells by stabilizing the virus capsid to block pH-dependent viral uncoating in endosomes and/or lysosomes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	FL cells
Concentration:	2xCGIC₅₀, 1xCGIC₅₀, CGIC₅₀/2, CGIC₅₀/4, CGIC₅₀/8 (CGIC₅₀ = 70 μmol/l)
Incubation Time:	48 h
Result:	Reduced viable cell number to 27.70% of control at 2xCGIC₅₀, 45.55% at 1xCGIC₅₀, 50.06% at CGIC₅₀/2, 59.04% at CGIC₅₀/4, and 68.50% at CGIC₅₀/8. Achieved a CGIC₅₀ of 70 μmol/l for FL cell growth inhibition. Yielded a selectivity index (SI = IC₅₀/CGIC₅₀) of 233.3, with an IC₅₀ of 0.3 μmol/l for poliovirus type 1 replication inhibition. Showed additive or sub-additive cytotoxic effects when combined with enviroxime, with no synergistic cytotoxicity detected.

Molecular Weight

342.43

Formula

C₂₀H₂₆N₂O₃

CAS No.

87495-31-6

Appearance

Solid

Color

White to off-white

SMILES

CC1=NOC(CCCCCCCOC2=CC=C(C3=NCCO3)C=C2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 3.33 mg/mL (9.72 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9203 mL	14.6015 mL	29.2030 mL
5 mM	0.5841 mL	2.9203 mL	5.8406 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Purity & Documentation

Purity: 96.50%

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Data Sheet (269 KB) SDS (254 KB)

COA (243 KB) HNMR (261 KB) RP-HPLC (264 KB) MS (230 KB)

Handling Instructions (2659 KB)

References

[1]. Nikolaeva L, et al. Cytotoxicity of the synergistic antienteroviral combination of enviroxime and disoxaril. Acta Virol. 1999 Aug;43(4):263-5. [Content Brief]

[2]. Zeichhardt H, et al. Inhibition of poliovirus uncoating by disoxaril (WIN 51711). Virology. 1987;160(1):281-285. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9203 mL	14.6015 mL	29.2030 mL	73.0076 mL
DMSO	5 mM	0.5841 mL	2.9203 mL	5.8406 mL	14.6015 mL

Disoxaril Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Disoxaril (Synonyms: WIN-51711)

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