2. Immunology/Inflammation
  3. CD276/B7-H3
  4. DS-5573a

DS-5573a is a human monoclonal antibody targeting B7-H3, with a Kd of 1.8 nM for the 4Ig isoform and 11 nM for the 2Ig isoform. DS-5573a induces antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis against B7-H3-expressing cancer cells. DS-5573a exerts dose-dependent anti-tumor activity via effector cells. DS-5573a can be used in the research of cancers including breast adenocarcinoma, non-small cell lung cancer, renal cell adenocarcinoma, gastric cancer and prostate cancer.

For research use only. We do not sell to patients.

DS-5573a

DS-5573a Chemical Structure

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Description

DS-5573a is a human monoclonal antibody targeting B7-H3, with a Kd of 1.8 nM for the 4Ig isoform and 11 nM for the 2Ig isoform. DS-5573a induces antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis against B7-H3-expressing cancer cells. DS-5573a exerts dose-dependent anti-tumor activity via effector cells. DS-5573a can be used in the research of cancers including breast adenocarcinoma, non-small cell lung cancer, renal cell adenocarcinoma, gastric cancer and prostate cancer[1].

Species Reactivity

Human
IC50 & Target

4IgB7-H3

1.8 nM (Kd)

2IgB7-H3

11 nM (Kd)

In Vitro

DS-5573a (1 μg/mL) binds to the IgC1 and IgC2 domains of human B7-H3 expressed in transfected CHO-K1 cells[1].
The Kd value of DS-5573a binding to recombinant human 4IgB7-H3 is 1.8 nM, and the Kd value of its binding to recombinant human 2IgB7-H3 is 11 nM[1].
DS-5573a (0.01-1000 ng/mL; 4 h) exerts potent, B7-H3-dependent antibody-dependent cell-mediated cytotoxicity against NCI-H322, MDA-MB-231 and COLO205 cells via human peripheral blood mononuclear cells (PBMC), while exhibits only minimal activity against B7-H3-negative CCRF-CEM cells[1].
DS-5573a (1-10000 ng/mL; 3 h) exerts B7-H3-dependent antibody-dependent cellular phagocytosis (ADCP) activity against NCI-H322, MDA-MB-231 and COLO205 cells via human monocyte-derived macrophages, but shows no activity against B7-H3-negative CCRF-CEM cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DS-5573a Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: NCI-H322, MDA-MB-231, and COLO205 cells
Concentration: 0.01, 0.1, 1, 10, 100, 1000 ng/mL
Incubation Time: 4 h
Result: Exerted potent, B7-H3-dependent ADCC activity against NCI-H322, MDA-MB-231, and COLO205 cells via human PBMCs.
In Vivo

DS-5573a (0.003-3 mg/kg; i.p.; once a week; 5 weeks) exhibits dose-dependent, statistically significant antitumor activity against MDA-MB-231 breast adenocarcinoma in female SCID mice, with significant efficacy observed at doses as low as 0.03 mg/kg and maximal activity at 0.3 mg/kg and 3 mg/kg[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CB17/Icr-Prkdcscid/CrlCrlj (female, breast adenocarcinoma model via s.c. inoculation of 5×106 MDA-MB-231 cells in Matrigel into right flank)[1]
Dosage: 0.003 mg/kg; 0.03 mg/kg; 0.3 mg/kg; 3 mg/kg
Administration: i.p.; once a week; 5 weeks
Result: Exhibited dose-dependent and statistically significant antitumor efficacy compared with vehicle-treated group: 0.03 mg/kg, 0.3 mg/kg, 3 mg/kg.
Reduced estimated tumor volumes in all treated groups relative to vehicle at 7 days after final treatment, with greatest reduction seen at 0.3 mg/kg and 3 mg/kg doses.
Gene ID

80381  [NCBI]

Accession
Target

B7-H3/CD276
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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DS-5573a Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DS-5573aCD276/B7-H3B7 homolog 3 proteinIgC1 extracellular domainhuman B7-H3renal cell adenocarcinomanon-small-cell lung cancerantibody-dependent cellular cytotoxicitybreast adenocarcinomaIgC2 extracellular domainNCI-H322 cellsantibody-dependent cellular phagocytosisCHO-K1 cellsInhibitorinhibitorinhibit

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