Ferroptosis/apoptosis inducer-4 

Ferroptosis/apoptosis inducer-4 is a pyridine-hydrazone-derived Cu (II) complex and a synergistic inducer of ferroptosis and apoptosis. Ferroptosis/apoptosis inducer-4 exerts anti-tumor proliferation and anti-metastasis effects with extremely low systemic toxicity. Ferroptosis/apoptosis inducer-4 disrupts cellular redox homeostasis by depleting glutathione and generating hydroxyl radicals through the Cu²⁺/Cu⁺ redox cycle. Ferroptosis/apoptosis inducer-4 also triggers mitochondrial dysfunction and endoplasmic reticulum stress, which in turn lead to Ca²⁺ release, mitochondrial Ca²⁺ overload, and the formation of a ROS−Ca²⁺ self-amplifying feedback loop. Ferroptosis/apoptosis inducer-4 can be used in studies related to cervical cancer^[1].

Ferroptosis/apoptosis inducer-4 (Cu2) (incubated for 24 h) potently inhibits the viability of HeLa, MCF-7, SK-OV-3, A549 and HCT-116 tumor cells, with IC₅₀s ranging from 0.39 μM to 1.44 μM, and exhibits low cytotoxicity against normal LX-2 cells^[1].
Ferroptosis/apoptosis inducer-4 (0.4-0.8 μM) induces caspase-dependent apoptosis in HeLa cells by altering the expression of pro-apoptotic and anti-apoptotic proteins, and activating caspase-3 and caspase-9 in a dose-dependent manner^[1].
Ferroptosis/apoptosis inducer-4 (0.4-0.8 μM) inhibits the migration and invasion of HeLa cells in a dose-dependent manner, and this effect is associated with the downregulated expression of MACC1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cu²⁺ (5 mg/kg; i.v.; every other day) exhibits superior in vivo antitumor efficacy against cervical cancer xenografts, with a 64.7% tumor growth inhibition rate, and demonstrates minimal systemic toxicity compared to cisplatin^[1].
Cu²⁺ (5 mg/kg; i.v.) effectively inhibits HeLa cell lung metastasis in nude mice, as evidenced by reduced lung metastatic burden and lower lung wet weight^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

481.65

C₁₄H₁₅Br₂CuFN₄

CC(C1=[N]2NC3=[N]([Cu+2]2([Br-])([N]4=C1C=CC=C4)[Br-])C=CC(F)=C3)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhang HQ, et al. Activation of Synergistic Ferroptosis and Apoptosis by a Cu(II) Complex through Concurrent Amplification of Endoplasmic Reticulum Stress and Mitochondrial Dysfunction. J Med Chem. 2026;69(9):10964-10978.