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Cat. No.: HY-187200

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SP-393D

SP-393D is an amidoxime-based prodrug inhibitor targeting dengue virus NS2B/NS3. The EC₅₀ values of SP-393D in Huh7 cells infected with DENV-1, DENV-2, DENV-3 and DENV-4 are 1.41, 0.066, 0.66 and 0.071 μM, respectively, while its CC₅₀ against Huh7 cells is >100 μM. SP-393D exhibits pan-serotypic activity against dengue virus serotypes 1, 2, 3 and 4. SP-393D binds to the allosteric pocket of dengue virus NS2B/NS3 protease and generates additional hydrogen bonding interactions. SP-393D can be used in studies related to dengue virus infection.

For research use only. We do not sell to patients.

Formula: C₂₈H₂₅BrN₈O₃
Molecular Weight:601.45

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

SP-393D Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

IC₅₀ & Target^[1]

DENV NS2B-NS3

In Vitro

SP-393D (compound 14a) (0.2-100 μM; 48 h) potently inhibits DENV2 replication in human hepatoma Huh7 cells, with an EC₅₀ of 0.06 μM; it also exhibits serotype-pan activity against DENV1, DENV3 and DENV4, with corresponding EC₅₀ values of 1.41 μM, 0.66 μM and 0.071 μM, respectively; and shows no cytotoxicity even at concentrations as high as 100 μM^[1].
The protonated active form of SP-393D binds to the allosteric site of the dengue virus (DENV) NS2B/NS3 protease-helicase complex and forms a variety of stable interactions, thereby inhibiting the protease function associated with viral replication^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 601.45
Formula C₂₈H₂₅BrN₈O₃
SMILES

O=C(NCC1=CC=C(/C(N)=N/O)C=C1)C2=CC(C3=CC(/C(N)=N/O)=CC=C3)=NC(N4CC(C=CC(Br)=C5)=C5C4)=N2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Kiousis D, et al. Design and synthesis of substituted pyrimidines with potent antiviral activity against dengue. European journal of medicinal chemistry. 2026 Jul 09;317:119130. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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