C12-NBD Sphinganine
Based on 1 Customer Validation
C12-NBD Sphinganine is a fluorescent ceramidase substrate. C12-NBD Sphinganine can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources.
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- Pureza: 99.9%
- No. CAS: 474943-05-0
- Fòrmula: C36H63N5O6
- Peso molecular:661.92
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Almacenamiento:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Actividad biológica
Chemical Information
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No. CAS 474943-05-0
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Appearance Solid
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Peso molecular 661.92
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Fòrmula C36H63N5O6
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Color Light yellow to yellow
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SMILES
CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(CCCCCCCCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvente y solubilidad
DMF : 30 mg/mL (45.32 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Rigogliuso S, et al. An active form of sphingosine kinase-1 is released in the extracellular medium as component of membrane vesicles shed by two human tumor cell lines. J Oncol. 2010;2010:509329. [Content Brief]
[2]. Okino N, et al. Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis. Biosci Biotechnol Biochem. 2010;74(2):316-21. [Content Brief]
[3]. Vijai Kumar Reddy T, et al. Design, synthesis and in vitro biological evaluation of short-chain C12-sphinganine and its 1,2,3-triazole analogs as potential antimicrobial and anti-biofilm agents. Eur J Med Chem. 2016 Aug 8;118:98-106. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMF | 1 mM | 1.5108 mL | 7.5538 mL | 15.1076 mL | 37.7689 mL |
| 5 mM | 0.3022 mL | 1.5108 mL | 3.0215 mL | 7.5538 mL | |
| 10 mM | 0.1511 mL | 0.7554 mL | 1.5108 mL | 3.7769 mL | |
| 15 mM | 0.1007 mL | 0.5036 mL | 1.0072 mL | 2.5179 mL | |
| 20 mM | 0.0755 mL | 0.3777 mL | 0.7554 mL | 1.8884 mL | |
| 25 mM | 0.0604 mL | 0.3022 mL | 0.6043 mL | 1.5108 mL | |
| 30 mM | 0.0504 mL | 0.2518 mL | 0.5036 mL | 1.2590 mL | |
| 40 mM | 0.0378 mL | 0.1888 mL | 0.3777 mL | 0.9442 mL |