ER-Laurdan
Based on 1 Customer Validation
ER-Laurdan is a derivative of the membrane-permeable fluorescent probe Laurdan (HY-D0080) that targets the endoplasmic reticulum (ER). As a membrane fluidity reporter, ER-Laurdan specifically localizes to the ER luminal membrane, and exhibits a prominent solvatochromic response to fluidity changes caused by membrane packing and exogenous saturated fatty acid stress. ER-Laurdan shows no overlapping localization with mitochondria, enables quantitative analysis of ER membrane fluidity via generalized polarization ratio measurement, and allows automatic signal masking with the help of ER markers. With high specificity and quantitative capability, ER-Laurdan serves as an important tool for investigating metabolic disorders and associated changes in the physical properties of cell membranes.
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- No. CAS: 3091879-77-2
- Fòrmula: C54H80F5N3O13
- Peso molecular:1074.22
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Almacenamiento:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
ER-Laurdan (5 μM; 20 min) localizes specifically to the endoplasmic reticulum (ER) in HeLa cells, with high colocalization with the ER marker ER-Tracker Red[1].
ER-Laurdan (5 μM; 20 min) detects reduced ER membrane fluidity (increased GP) in HeLa cells treated with 200 μM PA (24-48 h), while lower PA concentrations do not induce significant changes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:5 μM
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Incubation Time:20 min
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Result:Showed high signal overlap with ER-Tracker Red, with a Pearson’s correlation coefficient of 0.94.
Specifically stained ER tubules with no overlap with neighboring mitochondria.
Chemical Information
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No. CAS 3091879-77-2
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Appearance Solid
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Peso molecular 1074.22
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Fòrmula C54H80F5N3O13
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Color White to light yellow
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SMILES
O=C(C1=C(F)C(F)=C(F)C(F)=C1F)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCNC(CN(C)C2=CC=C3C(C=CC(C(CCCCCCCCCCC)=O)=C3)=C2)=O
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Structure Classification
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Pureza y Documentación
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Ficha de datos (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ER-Laurdan Related Classifications
HY-N16308 Related Classifications
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