HADA hydrochloride (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation^[2]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 250 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 250 μM with cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. S. aureus strains staining
2.1 Inoculate the S. aureus strain in a culture dish.
2.2 Add HADA hydrochloride (250 μM) to the cells.
2.3 Incubate cells at room temperature or 37°C for 30 min (optimized according to the experiment).
2.4 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.