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Cat. No.: HY-151381

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hDHODH-IN-10

hDHODH-IN-10 is a selective, potent and orally active hDHODH inhibitor, with an IC₅₀ value of 10.9 nM. hDHODH-IN-10 forms hydrogen bonds with key residues Arg136 and Gln47. hDHODH-IN-10 inhibits the proliferation of cancer cells. hDHODH-IN-10 can be used in the research of cancers, such as AML, colorectal cancer.

For research use only. We do not sell to patients.

CAS No.: 3033087-05-4
Formula: C₂₁H₁₅ClF₄N₂O₄
Molecular Weight:470.80

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

hDHODH-IN-10 Chemical Structure

CAS No.: 3033087-05-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: hDHODH (10.9 nM)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	0.25 μM Compound: 7d	Antiproliferative activity against human A-375 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human A-375 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
HCT-116	IC₅₀	0.14 μM Compound: 7d	Antiproliferative activity against human HCT-116 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human HCT-116 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
Kasumi 1	IC₅₀	0.1 μM Compound: 7d	Antiproliferative activity against human Kasumi 1 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human Kasumi 1 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
KG-1	IC₅₀	0.43 μM Compound: 7d	Antiproliferative activity against human KG-1 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human KG-1 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
L02	IC₅₀	1.1 μM Compound: 7d	Cytotoxicity against human HL7702 cells incubated for 96 hrs by MTT assay Cytotoxicity against human HL7702 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
NCM460	IC₅₀	14.2 μM Compound: 7d	Cytotoxicity against human NCM460 cells incubated for 96 hrs by MTT assay Cytotoxicity against human NCM460 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
Raji	IC₅₀	0.06 μM Compound: 7d	Antiproliferative activity against human Raji cells incubated for 96 hrs by MTT assay Antiproliferative activity against human Raji cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
SUD4	IC₅₀	0.75 μM Compound: 7d	Antiproliferative activity against human SU-DHL-4 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human SU-DHL-4 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]
U-937	IC₅₀	0.22 μM Compound: 7d	Antiproliferative activity against human U-937 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human U-937 cells incubated for 96 hrs by MTT assay	[PMID: 36115209]

In Vitro

hDHODH-IN-10 (compound 7d, 1 nM-100 μM) displays anti-proliferative activities against multiple human cancer cells^[1].
hDHODH-IN-10 (0.0625-0.25 μM, 24 h) increases the percentage of S-phase cells in Raji and HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

U937, HCT116, A375, Kasumi-1 and KG-1 cells
Concentration:

0-10 μM approximately
Incubation Time:

96 h
Result:

Inhibits cell proliferation with IC₅₀ values of 0.1-0.8 μM.

Cell Cycle Analysis^[1]

Cell Line:

Raji and HCT116 cells
Concentration:

0.0625, 0.125 and 0.25 μM
Incubation Time:

24 h
Result:

Increased the percentage of S-phase cells from 42.8% to 54.2%, 60.6% and 67.1%, respectively.

In Vivo

hDHODH-IN-10 (compound 7d, 30 mg/kg, oral administration) inhibits tumor growth in Raji and HCT116 cells xenograft mice model^[1].
hDHODH-IN-10 (500 mg/kg, oral administration) exhibits a favorable safety profile^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Raji and HCT116 cells xenograft mice model^[1]
Dosage:

30 mg/kg
Administration:

Oral administration
Result:

Showed a tumor growth inhibitory (TGI) rate of 58.3% (Raji model) and 42.1% (HCT116 model).

Animal Model:

BALB/c mice (acute toxicity assay) ^[1]
Dosage:

500 mg/kg
Administration:

Oral administration
Result:

LD₅₀ is about 500 mg/kg.
Induced a weak dysfunction of liver.

Chemical Information

CAS No. 3033087-05-4
Molecular Weight 470.80
Formula C₂₁H₁₅ClF₄N₂O₄
SMILES

C/C(O)=C(C(NC1=C(C=C(C(O[C@H](C(F)(F)F)C)=C1)C(C2=C(C=CC=C2)Cl)=O)F)=O)\C#N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chungen Li, et al. Discovery of potent human dihydroorotate dehydrogenase inhibitors based on a benzophenone scaffold. European Journal of Medicinal Chemistry, 2022, 243 (5), 114737.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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