2. Membrane Transporter/Ion Channel
  3. URAT1
  4. HYJ-2

HYJ-2 is a URAT1 inhibitor and urate-lowering agent. HYJ-2 inhibits URAT1-mediated urate transport and interacts with key residues within the URAT1 binding pocket. HYJ-2 reduces serum urate levels in hyperuricemic mice without inducing liver or kidney injury. HYJ-2 shows low cytotoxicity to hepatocytes and renal cells. HYJ-2 does not significantly induce hepatocyte apoptosis or mitochondrial dysfunction. HYJ-2 can be used in studies related to hyperuricemia and gout.

For research use only. We do not sell to patients.

HYJ-2

HYJ-2 Chemical Structure

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HYJ-2 Related Antibodies

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Description

HYJ-2 is a URAT1 inhibitor and urate-lowering agent. HYJ-2 inhibits URAT1-mediated urate transport and interacts with key residues within the URAT1 binding pocket. HYJ-2 reduces serum urate levels in hyperuricemic mice without inducing liver or kidney injury. HYJ-2 shows low cytotoxicity to hepatocytes and renal cells. HYJ-2 does not significantly induce hepatocyte apoptosis or mitochondrial dysfunction. HYJ-2 can be used in studies related to hyperuricemia and gout[1].

In Vitro

HYJ-2 (10-10000 nM) potently inhibits URAT1-mediated urate uptake in HEK293 cells overexpressing URAT1, with an IC50 of 368 nM[1].
HYJ-2 exhibits low cytotoxicity against HEK293T cells, with an IC50 of 353.1 μM[1].
HYJ-2 exhibits low cytotoxicity against HepG2 cells, with an IC50 value of 287.3 μM[1].
HYJ-2 (100 μM; 24 h) does not significantly promote apoptosis in HepG2 cells[1].
HYJ-2 (100 μM; 24 h) exerts weak effects on mitochondrial function in HepG2 cells, with 73.7% of cells retaining high mitochondrial membrane potential[1].
HYJ-2 exhibits excellent metabolic stability in human liver microsomes, with a half-life of 990 min, and 97.3% of the parent drug remains after 60 min of incubation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HYJ-2 Related Antibodies

Apoptosis Analysis[1]

Cell Line: human hepatocellular carcinoma HepG2 cells
Concentration: 100 μM
Incubation Time: 24 h
Result: Induced an apoptotic rate of 5.6% in HepG2 cells, which was comparable to the control group.
In Vivo

HYJ-2 (2 mg/kg; 19 days) reduces serum uric acid levels by 36.7% in hyperuricemic male Kunming mice, while alleviating hyperuricemia-related renal injury without inducing obvious liver injury[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice with Hyperuricemia (male, 19-21 g)[1]
Dosage: 2 mg/kg
Administration: 19 days
Result: Reduced mean serum uric acid levels from 1042.3 μM to 683.3 μM, corresponding to a 36.7% decrease rate.
Alleviated renal pathological injury associated with long-term hyperuricemia, with better preservation of renal tubular architecture and a more pronounced reduction in interstitial inflammatory cell infiltration than the reference drug group.
Showed no apparent hepatic pathological abnormalities.
Molecular Weight

322.15

Formula

C14H9Cl2N3O2
SMILES

CC1=CC=CC2=C(C(C3=CC(Cl)=C(O)C(Cl)=C3)=O)N=NN21
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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HYJ-2 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HYJ-2HYJ2HYJ 2URAT1Urate transporter 1SLC22A12hepatic cellshyperuricemic miceapoptosisgoutHEK293 cellsmitochondrial dysfunctionrenal cellsHepG2 cellshyperuricemiaInhibitorinhibitorinhibit

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