Cat. No.: HY-N0771

L-Isoleucine

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

L-Isoleucine is an orally active branched chain amino acid, which is the L-enantiomer of isoleucine. L-Isoleucine has a role as a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a plant metabolite, a human metabolite, an algal metabolite and a mouse metabolite. L-Isoleucine regulates the inflammatory response to protect against pathogens in vivo and in vitro.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 73-32-5
Formula: C₆H₁₃NO₂
Molecular Weight:131.18

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

L-Isoleucine Chemical Structure

CAS No.: 73-32-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

L-Isoleucine (0-16 mM, 12 h and 24 h) effectively relieves the decrease of TNF-α on cell viability in Intestinal epithelioid cells (IEC)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Intestinal epithelioid cell lines
Concentration:

0-16 mM
Incubation Time:

12 h, 24 h
Result:

Relieved the reduction of IEC-18 cell viability induced by TNF-a exposure in intestinal epithelioid cell lines.

In Vivo

L-Isoleucine [1.00% (w/w), p.o., 35 days] alleviates the growth performance impairment induced by dextran sulfate sodium (DSS), relieves the effect of DSS-induced colonic length shortage and the levels of IL-1β, IL-4, and IL-17 change in the colon of rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats with L-isoleucine-supplemented diet and L-alanine-supplemented diet ^[1]
Dosage:

1.00% (w/w)
Administration:

p.o., 35 d (measure per 3 days)
Result:

Alleviated DSS-induced growth performance impairment and delayed the DSS-induced DAI in rats.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 73-32-5
Appearance Solid
Molecular Weight 131.18
Formula C₆H₁₃NO₂
Color White to off-white
SMILES

N[C@@H]([C@@H](C)CC)C(O)=O
Structure Classification
- Amino acids
Initial Source
- Plants
- other families
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
J Nutr Biochem

Food-derived chlorogenic acid prevents aortic aneurysm and dissection by nutritional restore branched-chain amino acid dyshomeostasis. [Abstract]

2025 Dec 6:150:110211. PMID: 41360205
Molecules

Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]

2025 Mar 9;30(6):1224. PMID: 40142001
Amino Acids

Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]

2024 Sep 18;56(1):56. PMID: 39292313
Research Square Preprint

2021 Jul.
Laurea Magistrale in Biomedical Engineering, Politecnico di Milano

2019 Jun.

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (190.59 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.6234 mL

38.1170 mL

76.2340 mL

5 mM

1.5247 mL

7.6234 mL

15.2468 mL

10 mM

0.7623 mL

3.8117 mL

7.6234 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 10 mg/mL (76.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mao X, et al. l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats[J]. Front Immunol. 2022 Jan 11;12:817583. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	7.6234 mL	38.1170 mL	76.2340 mL	190.5851 mL
	5 mM	1.5247 mL	7.6234 mL	15.2468 mL	38.1170 mL
	10 mM	0.7623 mL	3.8117 mL	7.6234 mL	19.0585 mL
	15 mM	0.5082 mL	2.5411 mL	5.0823 mL	12.7057 mL
	20 mM	0.3812 mL	1.9059 mL	3.8117 mL	9.5293 mL
	25 mM	0.3049 mL	1.5247 mL	3.0494 mL	7.6234 mL
	30 mM	0.2541 mL	1.2706 mL	2.5411 mL	6.3528 mL
	40 mM	0.1906 mL	0.9529 mL	1.9059 mL	4.7646 mL
	50 mM	0.1525 mL	0.7623 mL	1.5247 mL	3.8117 mL
	60 mM	0.1271 mL	0.6353 mL	1.2706 mL	3.1764 mL
	80 mM	0.0953 mL	0.4765 mL	0.9529 mL	2.3823 mL
	100 mM	0.0762 mL	0.3812 mL	0.7623 mL	1.9059 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Isoleucine

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Publications Citing Use of MedChemExpress (MCE) L-Isoleucine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Isoleucine Related Classifications

Cat. No.: HY-N0771