2. Immunology/Inflammation
  3. Nuclear Factor of activated T Cells (NFAT)
  4. INCA-6

INCA-6  (Synonyms: Triptycene-1,4-quinone)

Cat. No.: HY-108544 Purity: 98.85%
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INCA-6 (Triptycene-1,4-quinone) is a cell-permeable NFAT inhibitor. INCA-6 specifically blocks targeting of NFAT(P) substrate to the  calcineurin (CN) phosphatase site and is an effective inhibitor of CN-NFAT signaling.

For research use only. We do not sell to patients.

INCA-6 Chemical Structure

INCA-6 Chemical Structure

CAS No. : 3519-82-2

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Based on 1 publication(s) in Google Scholar

INCA-6 Related Antibodies

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Description

INCA-6 (Triptycene-1,4-quinone) is a cell-permeable NFAT inhibitor. INCA-6 specifically blocks targeting of NFAT(P) substrate to the  calcineurin (CN) phosphatase site and is an effective inhibitor of CN-NFAT signaling[1][2][3].

In Vitro

INCA-6 (5 μM; for 24-hour) prevents transient outward K+ current (Ito) downregulation in 3-Hz cells[1].
Pre-treatment of BV-2 cells with INCA-6 (10 μM) significantly inhibits ATP-induced CXCL2 expression in BV-2 cells. INCA-6 also inhibits ATP-induced CXCL2 expression in rat primary microglia[2]. 
INCA-6 (5 μM) reduces SERCA2 transcript levels as well as protein expression, in the absence or in the presence of  thapsigargin (TG)[3].
INCA-6 (1.0 and 2.5 μM; 24 hours ) treatment significantly decreases both VEGF and serum-induced human retinal microvascular endothelial cells (HRMEC) proliferation, but does not affect baseline proliferation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

INCA-6 Related Antibodies

Cell Proliferation Assay[4]

Cell Line: Human retinal microvascular endothelial cells
Concentration: 0.5, 1.0, or 2.5 μM
Incubation Time: 24 hours
Result: Significantly inhibited VEGF-induced proliferation at 1.0 and 2.5 μM concentrations.
In Vivo

INCA-6 (5.0, or 25.0 μM) treatment significantly reduces pathologic neovascularization in oxygen-induced retinopathy (OIR)[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats bearing OIR model[4]
Dosage: 2.5, 5.0, or 25.0 μM
Administration: Intravitreal injection on days 14(0) and 14(3)
Result: Decreased the severity of OIR in a dose dependent manner. Significant inhibition was seen at 5.0 and 25.0 μM concentrations.
Molecular Weight

284.31

Formula

C20H12O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=CC(C2=C1C3C4=C(C2C5=C3C=CC=C5)C=CC=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 2.86 mg/mL (10.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5173 mL 17.5864 mL 35.1729 mL
5 mM 0.7035 mL 3.5173 mL 7.0346 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

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=
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C2

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V2

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Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5173 mL 17.5864 mL 35.1729 mL 87.9322 mL
5 mM 0.7035 mL 3.5173 mL 7.0346 mL 17.5864 mL
10 mM 0.3517 mL 1.7586 mL 3.5173 mL 8.7932 mL
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INCA-6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

INCA-63519-82-2Triptycene-1,4-quinoneINCA6INCA 6Nuclear Factor of activated T Cells (NFAT)NFATretinalmicrovascularendothelialoxygen-inducedretinopathytranscriptionfactorsneovascularizationInhibitorinhibitorinhibit

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