2. Metabolic Enzyme/Protease
  3. Dihydroorotate Dehydrogenase
  4. Indoluidin D

Indoluidin D is a selective dihydroorotate dehydrogenase (DHODH) inhibitor with a human DHODH IC50 of 210 nM. Indoluidin D selectively inhibits human DHODH activity, with induced effects rescuable by orotic acid. Indoluidin D promotes myeloid differentiation and inhibits cancer cell proliferation. Indoluidin D can be used for the research of acute promyelocytic leukemia.

For research use only. We do not sell to patients.

Indoluidin D

Indoluidin D Chemical Structure

CAS No. : 2763081-61-2

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Indoluidin D Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Indoluidin D is a selective dihydroorotate dehydrogenase (DHODH) inhibitor with a human DHODH IC50 of 210 nM. Indoluidin D selectively inhibits human DHODH activity, with induced effects rescuable by orotic acid. Indoluidin D promotes myeloid differentiation and inhibits cancer cell proliferation. Indoluidin D can be used for the research of acute promyelocytic leukemia[1].

In Vitro

Indoluidin D (0.01-1000 nM; 96 h) induces myeloid differentiation in human promyelocytic leukemia HL-60 cells with an EC50 of 10 nM, and this effect is specifically reversed by orotic acid[1].
Indoluidin D (72 h) inhibits growth of multiple human cancer cell lines, with the highest potency against acute T cell leukemia Jurkat cells (IC50 = 0.68 nM) and the lowest activity against normal pancreatic 1B2C6/1C3D3 and osteosarcoma Saos-2/MG-63 cells[1].
Indoluidin D (1-1000 nM; 30 min preincubation) inhibits recombinant human DHODH/ΔTM enzyme activity with an IC50 of 210 nM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Indoluidin D Related Antibodies

Cell Differentiation Assay[1]

Cell Line: human promyelocytic leukemia HL-60 cells
Concentration: 0, 10, 20, 30, 40 nM
Incubation Time: 96 h
Result: Potently induced myeloid differentiation of HL-60 cells, with an EC50 of 10 nM.
Induced expression of CD11b, a myeloid differentiation marker, in HL-60 cells.
Induced differentiation was rescued by addition of 1 mM orotic acid but not 1 mM dihydroorotic acid.

Cell Viability Assay[1]

Cell Line: human promyelocytic leukemia HL-60 cells
Concentration: 0.01, 0.1, 1, 10, 100, 1000 nM
Incubation Time: 72 h
Result: Potently suppressed viability and proliferation of HL-60 cells, with an IC50 of 4.4 nM.
Molecular Weight

452.55

Formula

C28H28N4O2
CAS No.
SMILES

O=C([C@H]1CC2=CC=CC=C2CN1)N[C@@H](C(NC3=CC=C(C=C3)C)=O)CC4=CNC5=CC=CC=C45
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Indoluidin D Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indoluidin D2763081-61-2Dihydroorotate DehydrogenaseDHODHHeLa cellsorotic aciddihydroorotate dehydrogenaseacute promyelocytic leukemiahuman DHODHmyeloid differentiationnormal pancreatic 1B2C6/1C3D3 cellshuman promyelocytic leukemia HL-60 cellsosteosarcoma Saos-2 cellsacute T cell leukemia Jurkat cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Indoluidin D
Cat. No.:
HY-182550
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.