Ponceau Staining Solution (Ready-to-use, 1×)
Based on 1 publication(s) in Google Scholar
MCE Ponceau S staining solution (ready-to-use, 1×) is a ready-to-use membrane staining solution that can be used for protein detection on PVDF membranes, nitrocellulose membranes, and cellulose acetate membranes.
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Storage :
Room temperature, 1 year
Description & Advantages
Ponceau S carries a negative charge and can bind to positively charged amino acid residues, as well as interact with the non-polar regions of proteins, forming red bands.
MCE Ponceau S staining solution (ready-to-use, 1×) is a ready-to-use membrane staining solution that can be used for protein detection on PVDF membranes, nitrocellulose membranes, and cellulose acetate membranes.
Ponceau S staining is reversible and can be washed off with distilled water, PBS, or other suitable solutions.
Protocol
1. Wash the membrane in ultrapure water 2 - 3 times for 1 min each time with gentle agitation to remove all transfer buffer.
2. Immerse the membrane in Ponceau Staining Solution, and then incubate with gentle agitation for 3 - 10 min.
3. Carefully wash the membrane in ultrapure water, PBS, other appropriate solutions 2 - 3 times for 3 - 5 min each time with gentle agitation to remove all ponceau staining solution.
Publications
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Journal Impact Factor
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Most Recent
Storage
Room temperature, 1 year
Attention
1. Ponceau staining solution is not suitable for protein detection on nylon membranes.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K1109-120 mL | HY-K1109-500 mL |
|---|---|---|
| Ponceau Staining Solution (Ready-to-use, 1×) | 120 mL | 500 mL |