BODIPY-Cholesterol
Based on 18 publication(s) in Google Scholar
BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 505/515 nm).
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 878557-19-8
- Formula: C36H51BF2N2O
- Molecular Weight:576.61
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BODIPY-Cholesterol
More- Signal Transduct Target Ther. 2025 May 23;10(1):171. [Abstract]
- Nat Microbiol. 2026 Jun;11(6):1677-1695. [Abstract]
- Autophagy. 2025 May;21(5):934-956. [Abstract]
- Nat Commun. 2026 Mar 11;17(1):3794. [Abstract]
- Exp Mol Med. 2025 May;57(5):1089-1105. [Abstract]
- Adv Sci (Weinh). 2026 Jun 17:e76144. [Abstract]
- Adv Healthc Mater. 2024 Apr;13(9):e2303337. [Abstract]
- Environ Int. 2024 Nov 26:194:109168. [Abstract]
- Adv Biotechnol (Singap). 2026 May 19;4(2):21. [Abstract]
- Clin Epigenetics. 2022 Dec 24;14(1):184. [Abstract]
- Front Pharmacol. 2023 Jun 26:14:1191692. [Abstract]
- ACS Infect Dis. 2024 May 10;10(5):1654-1663. [Abstract]
- Front Biosci (Landmark Ed). 2024 Sep 24;29(9):339. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2026 Jan;1871(1):159704. [Abstract]
- bioRxiv. 2026 May 20.
- bioRxiv. 2025 Aug 10.
- bioRxiv. 2025 Feb 17.
- bioRxiv. 2024 Feb 2.
Customer Validation & Images
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Cell Imaging/Staining
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[1]
1.1 Prepare stock solution
Solvent: DMSO
Concentration: 1 mM is recommended (e.g., weigh 0.58 mg BODIPY-Cholesterol and dissolve in 1 mL solvent).
Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 0.5-5 μM (optimized according to the experiment) with PBS (or other buffer).
Note: The working solution should be prepared and used immediately, and protected from light.
2. Cell staining
2.1 Wash cells with PBS.
2.2 Label the cell suspension with BODIPY-Cholesterol (final concentration 0.5-5 μM), mix well and incubate at 37°C for 10-20 minutes.
2.3 Flow cytometric detection (Ex/Em = 505/515 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
505
Chemical Information
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CAS No. 878557-19-8
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Appearance Solid
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Molecular Weight 576.61
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Formula C36H51BF2N2O
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Color Orange to reddish brown
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(C3=C(C)C=C(C)[N-]3[B+3]([F-])4[F-])=C5[N]4=C(C)C=C5C)([H])[C@@]6([H])[C@@](CC1)([H])[C@@]7(C(C[C@H](CC7)O)=CC6)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (18)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis. [Abstract]2025 May 23;10(1):171. PMID: 40404630 -
Nat Microbiol
Lipid accumulation in tuberculosis granulomas inhibits macrophage-CD4+ T cell interactions and infection control. [Abstract]2026 Jun;11(6):1677-1695. PMID: 41933199 -
Autophagy
2025 May;21(5):934-956. PMID: 39663580
BODIPY-Cholesterol purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May;21(5):934-956. [Abstract]
Representative confocal images of BODIPY-cholesterol (10 μg/mL; 24 h) staining after treatment of U2OS cells with indatraline (10 μM) or sertraline (10 μM) for 18 h were obtained.
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Nat Commun
A hierarchical theranostic nanoagent for multimodal imaging and targeted foam cell intervention in atherosclerosis. [Abstract]2026 Mar 11;17(1):3794. PMID: 41807467 -
Exp Mol Med
Cholesterol biosynthesis induced by radiotherapy inhibits cGAS-STING activation and contributes to colorectal cancer treatment resistance. [Abstract]2025 May;57(5):1089-1105. PMID: 40355720 -
Adv Sci (Weinh)
Cholesterol Laundry of Cell Membrane and Fatty Liver by Detergent Liposomes to Improve Anti-Cancer Drug Responsiveness of Patient Liver Tissues. [Abstract]2026 Jun 17:e76144. PMID: 42307256 -
Adv Healthc Mater
Nanointegrative Glycoengineering-Activated Necroptosis of Triple Negative Breast Cancer Stem Cells Enables Self-Amplifiable Immunotherapy for Systemic Tumor Rejection. [Abstract]2024 Apr;13(9):e2303337. PMID: 38154036 -
Environ Int
Cresyl diphenyl phosphate (a novel organophosphate ester) induces hepatic steatosis by directly binding to liver X receptor α: From molecule action to risk assessment. [Abstract]2024 Nov 26:194:109168. PMID: 39612745 -
Adv Biotechnol (Singap)
Phagocytotic impairment of tissue-resident alveolar macrophages by diesel particulates drives pulmonary surfactant accumulation. [Abstract]2026 May 19;4(2):21. PMID: 42154350 -
Clin Epigenetics
Identification of hepatocellular carcinoma subtypes based on PcG-related genes and biological relevance with cancer cells. [Abstract]2022 Dec 24;14(1):184. PMID: 36566204 -
Front Pharmacol
HECT, UBA and WWE domain containing 1 represses cholesterol efflux during CD4+ T cell activation in Sjögren's syndrome. [Abstract]2023 Jun 26:14:1191692. PMID: 37435494 -
ACS Infect Dis
MicroRNA-144-3p Inhibits Host Lipid Catabolism and Autophagy by Targeting PPARα and ABCA1 During Mycobacterium Tuberculosis Infection. [Abstract]2024 May 10;10(5):1654-1663. PMID: 38578697 -
Front Biosci (Landmark Ed)
CTRP9: An Anti-Atherosclerotic Factor in ApoE Knockout Mice through Oxidative Stress Inhibition. [Abstract]2024 Sep 24;29(9):339. PMID: 39344333 -
Biochim Biophys Acta Mol Cell Biol Lipids
Melatonin ameliorates ox-LDL-induced lipid accumulation and enhances ABCA1 expression in VSMCs via inhibition of the TRPV1-Ca2+-calpain signaling pathway. [Abstract]2026 Jan;1871(1):159704. PMID: 41213324 -
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Solvent & Solubility
DMSO : 1 mg/mL (1.73 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28. [Content Brief]
[2]. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7343 mL | 8.6714 mL | 17.3427 mL | 43.3569 mL |
BODIPY-Cholesterol Related Classifications
HY-125746 Related Classifications
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