Di-8-ANEPPS
Based on 1 publication(s) in Google Scholar
Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.84%
- CAS 番号: 157134-53-7
- 分子式: C36H52N2O3S
- 分子量:592.87
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保管条件:
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
MedChemExpress(MCE)の使用を引用している文献 Di-8-ANEPPS
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生物活性
A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the "center" signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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化学情報
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CAS 番号 157134-53-7
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性状 Solid
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分子量 592.87
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分子式 C36H52N2O3S
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Color Brown to red
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SMILES
O=S(CCC[N+]1=CC=C(/C=C/C2=CC=C3C=C(N(CCCCCCCC)CCCCCCCC)C=CC3=C2)C=C1)([O-])=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (1)
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Journal Impact Factor
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Most Recent
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Food Funct
2024 Sep 30;15(19):9863-9879. PMID: 39246047
溶剤 & 溶解度
DMSO : 1 mg/mL (1.69 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
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データシート (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6867 mL | 8.4336 mL | 16.8671 mL | 42.1678 mL |