Deoxyribonuclease II
Based on 2 publication(s) in Google Scholar
Deoxyribonuclease II (DNase II) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3' phosphate and 5'-hydroxyl termini. Deoxyribonuclease II works best at acidic pH and is commonly used in biochemical research.
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- CAS 番号: 9025-64-3
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
MedChemExpress(MCE)の使用を引用している文献 Deoxyribonuclease II
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生物活性
Product Information
Molecular Weight: 38.0 kDa
Optimal pH: 4.5-5.0
Activators: Cysteine, EDTA
Inhibitors: Actinomycin D, iodoacetate, N-bromosuccinimide, H2O2, divalent cations (such as zinc and copper), sulfate, NaCl (above 250 mM), DNA (above 4 mg/mL)
Instructions
Soluble in pure water (1 mg/mL), fresh preparation is recommended. Or dissolve in buffer according to specific experimental references.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.1.22.1
≥800 U/mg soild
One unit is defined as the amount of enzyme that can causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6.
化学情報
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CAS 番号 9025-64-3
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性状 Solid
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Color White to light yellow
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SMILES
[Deoxyribonuclease II]
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別名
デオキシリボヌクレアーゼII; DNase II
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
溶剤 & 溶解度
H2O : ≥ 5 mg/mL
* "≥" means soluble, but saturation unknown.
純度とドキュメンテーション
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データシート (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)