Agarose
Based on 2 publication(s) in Google Scholar
MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.
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Storage :
Store at room temperature for 3 years.
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Description & Advantages
Agarose is widely used for nucleic acid isolation and identification. This product can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.
| Features | |
|---|---|
| Gel Strength (1% gel) | > 1,200 g/cm2 |
| Electroosmosis (EEO) | < 0.15 |
| Sulfide | ≤ 0.15% |
| Gel Point (1.5% gel) | 35-37°C |
| Melting Point (1.5% gel) | 87-89°C |
| Water concentration | ≤ 10% |
| Nuclease | None |
プロトコル
1. Choose appropriate agarose concentration according to the size of nucleic acid fragment and the type of electrophoresis buffer.
2. Prepare the gel
a. Add appropriate agarose into the cleaned conical flask and dissolve with appropriate electrophoresis buffer (TAE or TBE). Seal the flask and heat in the microwave until the agarose is completely dissolved.
b. Add Nucleic Acid dye (HY-K1004 MCE SYBR Green I Nucleic Acid Gel Stain).
c. Mix gently and pour the agarose solution into the rubber board and insert the comb in place. The thickness of the gel is generally 3-5 mm.
d. Cool at room temperature for 30-60 minutes for subsequent electrophoresis.
Publications
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Journal Impact Factor
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Most Recent
保管条件
Store at room temperature for 3 years.
成分
| Components | HY-K1031-100 g |
|---|---|
| Agarose | 100 g |
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