KUL-7211

KUL 7211 (KUL-7211) is dissolved in distilled water with an equivalent molarity of HCl^[1].
Experiments are carried out using rat isolated atria, uterus, and proximal colon. Rats are stunned and then killed by rapid exsanguination. Each organ is rapidly removed and suspended in a 10 or 20 mL organ bath. The bath solution is Krebs solution (118.1 mM NaCl, 4.7 mM KCl, 2.5 mM CaCl₂, 1.2 mM MgSO₄• 7H₂O, 25.0 mM NaHCO₃, 1.2 mM KH₂PO₄, 11.1 mM glucose) in the atria and colon experiments and Locke-Ringer solution (154 mM NaCl, 5.6 mM KCl, 2.6 mM CaCl₂, 2.1 mM MgCl₂, 6.0 mM NaHCO₃, 2.8 mM glucose) in the uterus experiment. Each is continuously gassed with a mixture of 95% oxygen and 5% carbon dioxide at 37°C. The bath solutions contain 1 μM phentolamine, 0.5μM desipramine, and 3 0μ M hydrocortisone to block α-adrenoceptors and neuronal and extraneuronal catecholamine uptake, respectively. Spontaneous contractions are measured isometrically by means of a force-transducer and measuring system (TB-611T, AP-601G, and RPM-6004 or model 45196A, model 1829, and model 7903) connected to a thermowriting rectigraph. An initial resting tension of 5 mN is placed on each organ, and it is allowed to equilibrate for 1 h. Each preparation is exposed to only one drug^[1].

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