PTPA-QM

Guidelines (The following is our recommended experimental protocol. This protocol is for reference only; specific operations should be adjusted according to your actual needs.)
1. Preparation of PTPA-QM Working Solution
1.1 Preparation of Stock Solution
Prepare 1 mM PTPA-QM stock solution using DMSO. Aliquot and store at -20°C protected from light, avoiding repeated freeze-thaw cycles.
1.2 Preparation of Working Solution
Dilute the stock solution with PBS buffer to prepare 10 μM PTPA-QM working solution.
Note: Please adjust the working solution concentration according to your actual situation, and prepare fresh solution each time, protecting from light throughout the process.
2. Cell Staining
2.1 Cell Preparation
2.2 Add PTPA-QM working solution and incubate for 60 min.
2.3 Wash cells with PBS and detect them under a fluorescence microscope or flow cytometer.
3. Staining of sections
3.1 Wash brain tissue sections three times with PBS.
3.2 Add PTPA-QM working solution (2 μM) and incubate at room temperature in the dark for 3 min.
3.3 Wash stained brain tissue sections three times with DMSO:H2O = 1:1.

PTPA-QM (10 μM) is a highly efficient, selective, and photostable fluorescence probe for viscosity detection. Its fluorescence intensity in 99% glycerol is 22 times higher than that in pure DMSO, and it is minimally affected by biologically related substances. ^[1]
PTPA-QM (10 μM) exhibits viscosity-dependent fluorescence enhancement, with its fluorescence intensity increasing approximately 8-fold in high-viscosity glycerol compared to low-viscosity PBS; simultaneously, this probe possesses aggregation-induced emission properties^[3].
PTPA-QM (0.30-40.00 Mm, 24 h) shows low cytotoxicity to PC12 cells^[1].
PTPA-QM (10 μM, 1 h) can specifically bind to Aβ fibrils through various intermolecular interactions, and its fluorescence intensity increases approximately 2-fold after binding in cell-free experiments^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.