KB05-Biotin
KB05-Biotin is streptavidin-conjugated KB05 (HY-149461) that retains the site-specific cysteine covalent reactivity of KB05, enabling specific detection of target proteins via the biotin-streptavidin system. KB05-Biotin serves as a site-specific probe in ELISA-ABPP assays for screening electrophilic small molecules that bind to scout fragment-sensitive cysteines on target proteins in cell lysates, without the need for protein purification.
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- CAS No.: 2724919-70-2
- 화학식: C42H57BrN8O8S
- 분자량:913.92
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
KB05-Biotin (1 μM; 1 h) binds site-specifically to the probe-sensitive cysteine residues: C211 of recombinant wild-type NAGK, C185 of recombinant wild-type HPCAL1, and C516 of recombinant wild-type CDKN2AIP, all expressed in HEK293T cell lysates[1].
KB02-Biotin (1 μM; 1 h) binds site-specifically to the probe-sensitive cysteine C299 in recombinant wild-type AKR1B10 expressed in HEK293T cell lysates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2724919-70-2
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Appearance Oil
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분자량 913.92
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화학식 C42H57BrN8O8S
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SMILES
O=C(NCCOCCOCCOCCOCCN1N=NC(CCCCNC(C2=CC=C(N(C(C=C)=O)C3=CC=C(Br)C=C3)C=C2)=O)=C1)CCCC[C@H]4[C@]5([H])[C@](NC(N5)=O)([H])CS4
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : 100 mg/mL (109.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0942 mL | 5.4709 mL | 10.9419 mL | 27.3547 mL |
| 5 mM | 0.2188 mL | 1.0942 mL | 2.1884 mL | 5.4709 mL | |
| 10 mM | 0.1094 mL | 0.5471 mL | 1.0942 mL | 2.7355 mL | |
| 15 mM | 0.0729 mL | 0.3647 mL | 0.7295 mL | 1.8236 mL | |
| 20 mM | 0.0547 mL | 0.2735 mL | 0.5471 mL | 1.3677 mL | |
| 25 mM | 0.0438 mL | 0.2188 mL | 0.4377 mL | 1.0942 mL | |
| 30 mM | 0.0365 mL | 0.1824 mL | 0.3647 mL | 0.9118 mL | |
| 40 mM | 0.0274 mL | 0.1368 mL | 0.2735 mL | 0.6839 mL | |
| 50 mM | 0.0219 mL | 0.1094 mL | 0.2188 mL | 0.5471 mL | |
| 60 mM | 0.0182 mL | 0.0912 mL | 0.1824 mL | 0.4559 mL | |
| 80 mM | 0.0137 mL | 0.0684 mL | 0.1368 mL | 0.3419 mL | |
| 100 mM | 0.0109 mL | 0.0547 mL | 0.1094 mL | 0.2735 mL |
KB05-Biotin Related Classifications
HY-185414 Related Classifications
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