MitoTracker Green FM

Name: MitoTracker Green FM
Brand: MedChemExpress
SKU: HY-135056
Rating: 5.0 (24 reviews)

Cat. No.: HY-135056 Purity: 95.48%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Mito-Tracker Green is a green fluorescent dye that selectively accumulates in the mitochondrial matrix. MitoTracker Green FM covalently binds mitochondrial proteins by reacting with free mercaptan of cysteine residues, allowing staining of mitochondrial membrane potential independent of membrane potential. Excitation/emission wavelength 490/523 nm.

For research use only. We do not sell to patients.

CAS No. : 201860-17-5

Size	Price	Stock	Quantity
50 μg		In-stock	Estimated Time of Arrival: December 31

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Based on 24 publication(s) in Google Scholar

Other Forms of MitoTracker Green FM:

MitoTracker Green FM (solution) In-stock

24 Publications Citing Use of MCE MitoTracker Green FM

Flow Cytometry

In Vivo Imaging

Cell Imaging/Staining

: MitoTracker Green FM purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821. [Abstract]; Mitochondrial misfolded proteins were determined by MitoTracker Green FM (0.1 μM; 37 °C; 30 min). Fluorescence intensity measurements were obtained using a BD FACSverse flow cytometer.

: MitoTracker Green FM purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Aug 11;23(1):559. [Abstract]; (A) Schematic diagram of wound modeling and live imaging for in vivo observation of mitochondrial transfer (SHED labeled with MitoTracker Green FM). (B) Representative two-photon images of the 3D-reconstructed wound area on day 2 post-treatment. Scale bar: 50 μm. Quantitative analysis of the colocalization area proportion of red and green fluorescence (n = 3).

: MitoTracker Green FM purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 5;23(1):276. [Abstract]; Mitochondrial ROS analysis using MitoSOX Red and MitoTracker Green FM (100 nM; 37°C; 15 min) staining.

: MitoTracker Green FM purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Dec 24;17(51):69180-69195. [Abstract]; (A) Fluorescence images of mitochondria and two nanocomposites (L@H–MnO2@AOAA and TPP-L@H–MnO2@AOAA) in LPS (1 μg/mL; 24 h)-activated RAW264.7 cells (scale bar, 10 μm). Green, mitochondria stained by MitoTracker Green FM (200 nM; 30 min); red, nanocomposites labeled by Cy3. Colocalization analysis of mitochondria with (B) L@H–MnO2@AOAA and (C)TPP-L@H–MnO2@AOAA.

: MitoTracker Green FM purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Apr 24;14(4):287. [Abstract]; K562G and K562R cells were stained with the MitoTracker Green (0.1 μM; 37 °C; 30 min) and then cells were subjected to flow cytometry assays for assessing mitochondrial number.

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Description

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[2].
1. Preparation of MitoTracker Green FM working solution
1.1 Preparation of the stock solution
Dissolve MitoTracker Green FM in DMSO to obtain 1 mM of stock solution.
1.2 Preparation of MitoTracker Green FM working solution
Dilute the stock solution in serum-free cell culture medium or PBS dilute to obtain 20-200 nM of working solution.
Note: Please adjust the concentration of MitoTracker Green FM working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL.
2.2. Add 1 mL of working solution, and then incubate at room temperature for 15-45 minutes.
2.3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4. Wash twice with PBS, 5 minutes each time.
2.5. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 15-45 minutes.
3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

671.87

Formula

C₃₄H₂₈Cl₅N₃O

CAS No.

201860-17-5

Appearance

Solid

Color

Yellow to orange

Emission (Em)

523

Excitation (Ex)

490

SMILES

C[N+]1=C(/C=C/C=C2N(CC3=CC=C(CCl)C=C3)C4=CC(Cl)=C(Cl)C=C4N\2CC5=CC=C(CCl)C=C5)OC6=CC=CC=C16.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (7.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4884 mL	7.4419 mL	14.8838 mL
5 mM	0.2977 mL	1.4884 mL	2.9768 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥99.0%

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Data Sheet (278 KB) SDS (476 KB)

COA (254 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: MitoTracker Green FM can be used to label cells with green fluorescence.
Method: For cell labeling.
1. Incubate cells with LPS (10.0 μg/mL; 24 h).
2. Stain cells with MitoTracker Green FM (500 nM; 30 min).
3. Use a Nikon C2 confocal laser scanning microscope for image.

References

[1]. Gautam N, et, al. A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization. Methods. 2018 Feb 1;134-135:11-19. [Content Brief]

[2]. Gautam N, et, al. A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization. Methods. 2018 Feb 1;134-135:11-19. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4884 mL	7.4419 mL	14.8838 mL	37.2096 mL
DMSO	5 mM	0.2977 mL	1.4884 mL	2.9768 mL	7.4419 mL