PTPA-QM
PTPA-QM is a low-cytotoxicity Aβ protein probe that can be used for live-cell imaging and tissue section staining (Lys. Ex/Em = 385/615 nm). PTPA-QM can intercalate into the β-sheet layered structure of β-amyloid fibrils, form intermolecular interactions with amino acid residues, restrict intramolecular rotation and trigger fluorescence activation for imaging purposes. PTPA-QM is applicable to Alzheimer's disease-related research. Maximum excitation/emission wavelength: 448/605 nm.
For research use only. We do not sell to patients.
- Formula: C38H27N5
- Molecular Weight:553.65
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (The following is our recommended experimental protocol. This protocol is for reference only; specific operations should be adjusted according to your actual needs.)
1. Preparation of PTPA-QM Working Solution
1.1 Preparation of Stock Solution
Prepare 1 mM PTPA-QM stock solution using DMSO. Aliquot and store at -20°C protected from light, avoiding repeated freeze-thaw cycles.
1.2 Preparation of Working Solution
Dilute the stock solution with PBS buffer to prepare 10 μM PTPA-QM working solution.
Note: Please adjust the working solution concentration according to your actual situation, and prepare fresh solution each time, protecting from light throughout the process.
2. Cell Staining
2.1 Cell Preparation
2.2 Add PTPA-QM working solution and incubate for 60 min.
2.3 Wash cells with PBS and detect them under a fluorescence microscope or flow cytometer.
3. Staining of sections
3.1 Wash brain tissue sections three times with PBS.
3.2 Add PTPA-QM working solution (2 μM) and incubate at room temperature in the dark for 3 min.
3.3 Wash stained brain tissue sections three times with DMSO:H2O = 1:1.
PTPA-QM (10 μM) is a highly efficient, selective, and photostable fluorescence probe for viscosity detection. Its fluorescence intensity in 99% glycerol is 22 times higher than that in pure DMSO, and it is minimally affected by biologically related substances. [1]
PTPA-QM (10 μM) exhibits viscosity-dependent fluorescence enhancement, with its fluorescence intensity increasing approximately 8-fold in high-viscosity glycerol compared to low-viscosity PBS; simultaneously, this probe possesses aggregation-induced emission properties[3].
PTPA-QM (0.30-40.00 Mm, 24 h) shows low cytotoxicity to PC12 cells[1].
PTPA-QM (10 μM, 1 h) can specifically bind to Aβ fibrils through various intermolecular interactions, and its fluorescence intensity increases approximately 2-fold after binding in cell-free experiments[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC12 cells
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Concentration:10 μM
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Incubation Time:1 h
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Result:Produced strong red fluorescence visible via CLSM after incubation for 1 h, indicating successful cell penetration and intracellular fluorescence imaging.
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Cell Line:PC12 cells
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Concentration:0.3 μM, 0.6 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM
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Incubation Time:24 h
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Result:Exhibited low cytotoxicity to PC12 cells.
Chemical Information
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Molecular Weight 553.65
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Formula C38H27N5
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SMILES
CN(C1=C/2C=CC=C1)C(/C=C/C(C=C3)=CC=C3N(C4=CC=CC=C4)C(C=C5)=CC=C5C6=CC=NC=C6)=CC2=C(C#N)/C#N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Fang Y, et al. A Novel Aggregation-Induced Emission Fluorescent Probe for Detection of β-Amyloid Based on Pyridinyltriphenylamine and Quinoline-Malononitrile. Biosensors (Basel). 2023;13(6):610. Published 2023 Jun 2. [Content Brief]
[2]. Zheng M, et al. Fluorescent Materials with Excellent Biocompatibility and Their Application in Bio-Sensing, Bio-Imaging. Biosensors (Basel). 2023;13(10):906. Published 2023 Sep 26. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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