Cyclovirobuxine D Inhibits Cell Proliferation and Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer Cells

  • Molecules. 2015 Nov 19;20(11):20659-68. doi: 10.3390/molecules201119729.
Jie Wu  1  2 ,  Zhujun Tan  3 ,  Jian Chen  4 ,  Cheng Dong  5  6
Affiliations
  • 1. School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. [email protected].
  • 2. Ministry of Education of China (MOE) Key Laboratory of Hydrodynamics, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. [email protected].
  • 3. Department of General Surgery, School of Medicine, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, Shanghai 200092, China. [email protected].
  • 4. School of Pharmacy, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. [email protected].
  • 5. School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. [email protected].
  • 6. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA. [email protected].
PMID: 26610442 DOI: 10.3390/molecules201119729
Abstract

Gastric Cancer is one of the most common malignant cancers, with high death rates, poor prognosis and limited treatment methods. Cyclovirobuxine D (CVB-D) is the main active component of the traditional Chinese medicine Buxus microphylla. In the present study, we test the effects of CVB-D on gastric Cancer cells and the underlying mechanisms of action. CVB-D reduced cell viability and colony formation ability of MGC-803 and MKN28 cells in a time- and concentration-dependent manner. Flow cytometry showed that cell cycle of CVB-D treated cells was arrested at the S-phase. CVB-D also induced Apoptosis in MGC-803 and MKN28 cells, especially early stage Apoptosis. Furthermore, mitochondria membrane potential (Δψm) was reduced and apoptosis-related proteins, cleaved Caspase-3 and Bax/Bcl-2, were up-regulated in CVB-D-treated MGC-803 and MKN28 cells. Taken together, our studies found that CVB-D plays important roles in inhibition of gastric tumorigenesis via arresting cell cycle and inducing mitochondria-mediated Apoptosis, suggesting the potential application of CVB-D in gastric Cancer therapy.

Keywords
apoptosis; cell cycle; cyclovirobuxine D; gastric cancer; mitochondria.
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