2. Apoptosis Autophagy PI3K/Akt/mTOR
  3. Apoptosis Autophagy mTOR Akt
  4. Cyclovirobuxine D

Cyclovirobuxine D 

Cat. No.: HY-N0107 Purity: ≥98.0%
COA Handling Instructions

Cyclovirobuxine D (CVB-D) is the main active component of the traditional Chinese medicine Buxus microphylla. Cyclovirobuxine D induces autophagy and attenuates the phosphorylation of Akt and mTOR. Cyclovirobuxine D inhibits cell proliferation of gastric cancer cells through suppression of cell cycle progression and inducement of mitochondria-mediated apoptosis. Cyclovirobuxine D is beneficial for heart failure induced by myocardial infarction.

For research use only. We do not sell to patients.

Cyclovirobuxine D Chemical Structure

Cyclovirobuxine D Chemical Structure

CAS No. : 860-79-7

Size Price Stock Quantity
Solution
10 mM * 1 mL in Ethanol USD 61 In-stock
Solid
5 mg USD 55 In-stock
10 mg USD 88 In-stock
20 mg USD 154 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Cyclovirobuxine D Related Antibodies

Top Publications Citing Use of Products

View All mTOR Isoform Specific Products:

View All Isoforms
mTOR mTORC1 mTORC2

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cyclovirobuxine D (CVB-D) is the main active component of the traditional Chinese medicine Buxus microphylla. Cyclovirobuxine D induces autophagy and attenuates the phosphorylation of Akt and mTOR[1]. Cyclovirobuxine D inhibits cell proliferation of gastric cancer cells through suppression of cell cycle progression and inducement of mitochondria-mediated apoptosis[2]. Cyclovirobuxine D is beneficial for heart failure induced by myocardial infarction[3].

In Vitro

Cyclovirobuxine D (0-240 µM ;24-72 hours) shows a concentration- and time-dependent reduced cell viability after CVB-D treatment, only 10% MGC-803 cells and 20% MKN28 cells are alive at 72 h after treatment with 240 µM[2].
Cyclovirobuxine D (0-120 µM; 48 hours) arrests the cell cycle of gastric cancer cells at S phase in a concentration-dependent manner[2].
Cyclovirobuxine D (0-120 µM; 48 hours) leads to apoptosis in gastric cancer cells in a concentration-dependent manner, especially early stage apoptosis. Cyclovirobuxine D (0-120 µM; 48 hours) causes apoptosis via up-regulation of the apoptosis- related proteins, cleaved Caspase-3 and ratio of Bax/Bcl-2, in gastric cancer cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclovirobuxine D Related Antibodies

Cell Viability Assay[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, 120 and 240 µM
Incubation Time: 24, 48, 72 hours
Result: Reduced Cell Viability and Colony Formation Ability of Gastric Cancer Cells

Cell Cycle Analysis[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, and 120 µM
Incubation Time: 48 hours
Result: Arrested cell cycle progressions of MGC-803 and MKN28 cells.

Apoptosis Analysis[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, and 120 µM
Incubation Time: 48 hours
Result: Induced apoptosis of MGC-803 and MKN28 cells.

Western Blot Analysis[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, and 120 µM
Incubation Time: 48 hours
Result: Up-regulated cleaved Caspase-3 and Bax and decreased the expression of Bcl-2 expression.
Molecular Weight

402.66

Appearance

Solid

Formula

C26H46N2O
CAS No.
SMILES

C[C@H](NC)[C@@]1([H])[C@H](O)C[C@@]2(C)[C@]3([H])CC[C@@]4([H])C(C)(C)[C@@H](NC)CC[C@]4(C5)[C@]35CC[C@@]21C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

Ethanol : 13 mg/mL (32.29 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4174 mL 24.8348 mL
5 mM 0.4967 mL 2.4835 mL 4.9670 mL
10 mM 0.2483 mL 1.2417 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.08 mg/mL (2.68 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.08 mg/mL (2.68 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 1.08 mg/mL (2.68 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (252 KB) HNMR (257 KB)

Handling Instructions (2659 KB)
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Cyclovirobuxine D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Cyclovirobuxine D860-79-7ApoptosisAutophagymTORAktMammalian target of RapamycinPKBProtein kinase BInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Cyclovirobuxine D
Cat. No.:
HY-N0107
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.