Synthesis of the biotinylated anti-HIV compound BMMP and the target identification study

  • Bioorg Med Chem Lett. 2016 Jan 1;26(1):43-5. doi: 10.1016/j.bmcl.2015.11.036.
Masahiro Kamo  1 ,  Hiroshi Tateishi  2 ,  Ryoko Koga  3 ,  Yoshinari Okamoto  4 ,  Masami Otsuka  5 ,  Mikako Fujita  6
Affiliations
  • 1. Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: [email protected].
  • 2. Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: [email protected].
  • 3. Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: [email protected].
  • 4. Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: [email protected].
  • 5. Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: [email protected].
  • 6. Research Institute for Drug Discovery, School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: [email protected].
PMID: 26615887 DOI: 10.1016/j.bmcl.2015.11.036
Abstract

BMMP [2-(benzothiazol-2-ylmethylthio)-4-methylpyrimidine], an inhibitor of HIV-1 replication, was linked to biotin to study the interaction with the presumed target, HIV-1 Pr55(Gag) or CA, by means of surface plasmon resonance. The synthesized Biotin-BMMP inhibited HIV-1 replication to a similar extent as BMMP alone, but did not interact with Pr55(Gag) or CA, suggesting that BMMP exerts its activity by a different mechanism.

Keywords
BMMP; Biotin; CA; HIV-1; Pr55(Gag).
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